Purified Rat Anti-Mouse IgG2a/2b

BD Pharmingen™ Purified Rat Anti-Mouse IgG2a/2b

Name: Purified Rat Anti-Mouse IgG2a/2b
Brand: BD Pharmingen™
SKU: 553397

Clone R2-40

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: IgG2a (Ighg2a; Igh-1); IgG2b (Ighg2b; Igh-3; gamma2b)

Reactivity: Mouse (QC Testing)

Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ

Immunogen: Pooled Mouse Ig

Application: ELISA (Routinely Tested)

Concentration: 0.5 mg/ml

Entrez Gene ID: 380793,380793, 16016

RRID: AB_394835

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Purified Rat Anti-Mouse IgG2a/2b may be used as a primary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2a/2b, we recommend FITC Rat Anti-Mouse IgG2a/2b (Cat. No. 553399).

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

553397 Rev. 15

Antibody Details

R2-40

The R2-40 monoclonal antibody specifically recognizes a common epitope, probably located in the CH1 domain, shared by mouse IgG2a, and IgG2b, of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes.

553397 Rev. 15

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

553397 Rev.15

Citations & References

Development References (1)

Benson MJ, Dillon SR, Castigli E, et al. Cutting edge: the dependence of plasma cells and independence of memory B cells on BAFF and APRIL.. J Immunol. 2008; 180(6):3655-9. (Biology). View Reference

553397 Rev. 15

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.