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PerCP-Cy5.5 Rat Anti-Mouse CD39
PerCP-Cy5.5 Rat Anti-Mouse CD39

Flow cytometric analysis of CD39 expression on mouse splenic lymphocytes. BALB/c mouse splenocytes were stained with BD Horizon™ BUV395 Rat Anti-Mouse CD4 (Cat. No. 563790/565974) and APC Rat Anti-Mouse CD25 (Cat. No. 557192/561048) antibodies, and with either PerCP-Cy5.5 Rat IgG2b, κ Isotype Control (Cat. No. 550764; Left Plots) or PerCP-Cy5.5 Rat Anti-Mouse CD39 antibody (Cat. No. 567270; Right Plots) at 0.125 μg/test. 7-AAD (7-Amino-Actinomycin D) Solution (Cat. No. 559925) was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

   Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes.

   Lower Plots: Bivariate pseudocolor density plots showing the correlated expression CD39 (or Ig Isotype control staining) versus CD25 were derived from CD4-positive gated events with the light scatter characteristics of viable (7-AAD-negative) lymphocytes.

Flow cytometric analysis of CD39 expression on mouse splenic lymphocytes. BALB/c mouse splenocytes were stained with BD Horizon™ BUV395 Rat Anti-Mouse CD4 (Cat. No. 563790/565974) and APC Rat Anti-Mouse CD25 (Cat. No. 557192/561048) antibodies, and with either PerCP-Cy5.5 Rat IgG2b, κ Isotype Control (Cat. No. 550764; Left Plots) or PerCP-Cy5.5 Rat Anti-Mouse CD39 antibody (Cat. No. 567270; Right Plots) at 0.125 μg/test. 7-AAD (7-Amino-Actinomycin D) Solution (Cat. No. 559925) was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

   Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes.

   Lower Plots: Bivariate pseudocolor density plots showing the correlated expression CD39 (or Ig Isotype control staining) versus CD25 were derived from CD4-positive gated events with the light scatter characteristics of viable (7-AAD-negative) lymphocytes.

Product Details
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BD Pharmingen™
ectonucleoside triphosphate diphosphohydrolase 1; Entpd1; ecto-ATP diphosphohydrolase 1; ecto-ATPDase 1; ecto-ATPase 1; Cd39; NTPDase 1
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2b, κ
Mouse CD39 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  6. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
  7. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
  10. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
567270 Rev. 1
Antibody Details
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Y23-1185

The Y23-1185 monoclonal antibody specifically recognizes mouse CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), which is an enzyme on the surface of vascular endothelial cells, antigen presenting cells and activated immune cells. CD39 is encoded by ENTPD1 which belongs to the ectoenzyme family. The catalytic portion of the CD39 is extracellular, where it acts on extracellular nucleoside triphosphates and diphosphates, including ATP and ADP that are hydrolyzed into AMP. Through cell surface CD73 (Ecto-5'-nucleotidase), regulatory T cells can act on extracellular AMP to generate immunosuppressive adenosine. CD39 is involved in the control of the extracellular pool of phosphorylated nucleosides, the suppression of inflammation and immunity, and the regulation of platelet activation.

567270 Rev. 1
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
567270 Rev.1
Citations & References
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Development References (5)

  1. Allard D, Allard B, Stagg J. On the mechanism of anti-CD39 immune checkpoint therapy. J Immunother Cancer. 2020; 8(1):e000186. (Biology). View Reference
  2. Borsellino G, Kleinewietfeld M, Di Mitri D, et al. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.. Blood. 2007. (Biology). View Reference
  3. Mizumoto N, Kumamoto T, Robson SC, et al. CD39 is the dominant Langerhans cell-associated ecto-NTPDase: modulatory roles in inflammation and immune responsiveness.. Nat Med. 2002; 8(4):358-365. (Biology). View Reference
  4. Salmi M, Jalkanen S. Ectoenzymes controlling leukocyte traffic.. Eur J Immunol. 2012; 42(2):284-92. (Biology). View Reference
  5. Zhou Q, Yan J, Putheti P, et al. 2009; 9(10):2303-2311. (Biology). View Reference
View All (5) View Less
567270 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.