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Rainbow Calibration Particles (8 peaks), 3.0 - 3.4 µm

Rainbow Calibration Particles (8 peaks), 3.0 - 3.4 µm

(RUO)
Rainbow Calibration Particles (8 peaks), 3.0 - 3.4 µm
Upper Left: Rainbow Particle peak separation based on FL1 fluorescence intensity expressed in relative channel numbers. Upper Center: Molecules of Equivalent Cy5™ (MECY) versus relative channel number for the 8 peaks present in the Rainbow Particles. Upper Right: Molecules of Equivalent Phycoerythrin (MEPE) versus relative channel number for the 8 peaks present in the Rainbow Particles. Lower Left: Molecules of Equivalent Fluorescein (MEFL) versus relative channel number for the 8 peaks present in the Rainbow Particles. Lower Middle: Molecules of Equivalent Phycoerythrin-TR (MEPTR) versus relative channel number for the 8 peaks present in the Rainbow Particles. Lower Right: Molecules of Equivalent APC (MEAP) versus relative channel number for the 8 peaks present in the Rainbow Particles.
Upper Left: Rainbow Particle peak separation based on FL1 fluorescence intensity expressed in relative channel numbers. Upper Center: Molecules of Equivalent Cy5™ (MECY) versus relative channel number for the 8 peaks present in the Rainbow Particles. Upper Right: Molecules of Equivalent Phycoerythrin (MEPE) versus relative channel number for the 8 peaks present in the Rainbow Particles. Lower Left: Molecules of Equivalent Fluorescein (MEFL) versus relative channel number for the 8 peaks present in the Rainbow Particles. Lower Middle: Molecules of Equivalent Phycoerythrin-TR (MEPTR) versus relative channel number for the 8 peaks present in the Rainbow Particles. Lower Right: Molecules of Equivalent APC (MEAP) versus relative channel number for the 8 peaks present in the Rainbow Particles.
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Description

The vial contains a mixture of 3.0 - 3.4 µm Rainbow Particles that are dyed to eight different fluorescent intensities. Every Rainbow Particle contains a mixture of fluorophores that are excited at any wavelength from 365 - 650 nm. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.



Preparation And Storage

Preparation and StorageStore undiluted at 2-8 °C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This particle mixture (~10x10^6 particles/ml) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Cy is a trademark of GE Healthcare.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559123 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.