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Fixation/Permeabilization Solution Kit with BD GolgiStop™

Fixation/Permeabilization Solution Kit with BD GolgiStop™

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Product Details
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BD Cytofix/Cytoperm™ Plus
RUO
AB_2869009


Description

The BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides Fixation/Permeabilization solution, BD Perm/Wash™ Buffer, and BD GolgiStop™ protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals. Enough BD GolgiStop reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10 cells/ml.

Components Quantity  Fixation/Permeabilization solution  125 mL  BD Perm/Wash™ Buffer (10x)  100 mL  BD GolgiStop protein transport inhibitor containing monensin  0.7 mL  

554715 Rev. 1
Components
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Description Quantity/Size Part Number
Fixation/Permeabilization solution 125 mL (1 ea) 51-2090KZ
BD Perm/Wash™ Buffer 100 mL (1 ea) 51-2091KZ
Protein Transport Inhibitor (contianing Monensin) 1 Each (1 ea) 51-2092KZ
554715 Rev. 1
Citations & References
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Development References (14)

  1. Alaverdi, N and JB Waters . Enhanced detection of antigens by flow cytometry with the BD Cytofix/Cytoperm™ intracellular staining kit. Hotlines. 3:6,7,15,16. (Biology).
  2. Anderson SJ, Coleclough C. Regulation of CD4 and CD8 expression on mouse T cells. Active removal from the cell surface by two mechanisms. J Immunol. 1993; 151(10):5123-5134. (Biology). View Reference
  3. Andersson J, Abrams J, Bjork L, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994; 83(1):16-24. (Biology). View Reference
  4. Andersson, U and J Andersson. D Fradelizie and D Emelie, ed. Cytokine Producing Cells. Paris: INSERM; 1994:32-49.
  5. Assenmacher M, J Schmitz and A Radbruch. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: Expression of interleukin-10 in interferon-γ and in interleukin-4-expressing cells. Eur J Immunol. 1994; 24:1097-1101. (Biology).
  6. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation. J Immunol. 1995; 154(9):4294-4301. (Biology). View Reference
  7. Ferrick DA, Schrenzel MD, Mulvania T, Hsieh B, Ferlin WG, Lepper H. Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo. Nature. 1995; 373(6511):255-257. (Biology). View Reference
  8. Jung T, Schauer U, Heusser C, Neumann C, Rieger C. Detection of intracellular cytokines by flow cytometry. J Immunol Methods. 1993; 159(1-2):197-207. (Biology). View Reference
  9. Litton M, Andersson J, Bjork L, Fehniger T, Ulfgren AK, Andersson U. Cytoplasmic cytokine staining in individual cells. In: Debets and Savelkoul, ed. Human Cytokine Protocols. Humana Press; 1996.
  10. Litton MJ, Sander B, Murphy E, O'Garra A, Abrams JS. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods. 1994; 175(1):47-58. (Biology). View Reference
  11. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). View Reference
  12. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Biology). View Reference
  13. Sander, B, J Andersson and U Andersson. Assessment of cytokines by immunofluorescence and the paraformalde-hyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Biology).
  14. Vikingsson A, Pederson K, Muller D. Enumeration of IFN-gamma producing lymphocytes by flow cytometry and correlation with quantitative measurement of IFN-gamma. J Immunol Methods. 1994; 173(2):219-228. (Biology). View Reference
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554715 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.