Fluorochrome marker assignment for a 17-color spectral flow cytometry panel
| Specificity | Clone | Fluorochrome | |
|---|---|---|---|
| UV 355 nm | CD25 | M-A251 | |
CD56 | NCAM16.2 | ||
CD20 | L27 | ||
CD19 | SJ25C1 | ||
CD16 | 3G8 | ||
CD14 | M5E2 | ||
AutoF | N/A | N/A | |
TIM-3 | 7D3 | ||
CD4 | SK3 | ||
| Violet 405 nm | TNF | MAb11 | |
VPD450 | N/A | N/A | |
CD3 | UCHT1 | ||
CD134 | ACT35 | ||
| Blue 488 nm | IFNγ | B27 | |
CD69 | FN50 | ||
FVS620 | N/A | N/A | |
LAG-3 | T47-530 | ||
PD-1 | EH12.1 | ||
II-2 | MQ1-17H12 | ||
| Yellow-Green 561 nm | CTLA-4 | BNI3 | |
| Red 640 nm | GranzB | GB11 | |
CD8 | SK1 |
Fluorochrome marker assignment for a 17-color spectral flow cytometry panel acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.