Description
The Mouse γ1 (IgG1) antibody, clone X40, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH. The CD3 antibody, clone SK7, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. The Mouse γ1 (IgG1) antibody recognizes a keyhole limpet hemocyanin (KLH) antigen not expressed on human cells. The CD3 antibody recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kilodaltons (kDa). The antigen recognized by the CD3 antibody is non-covalently associated with either α/β or γ/δ TCR (70 to 90 kDa).
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| Mouse IgG1 Isotype Control PE | L293 | IgG1, κ | N/A |
| CD3 PerCP | SK7 | IgG1, κ | N/A |
Development References (13)
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Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Kan EA, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with anti-Leu-4 antibody. J Immunol. 1983; 131(2):536-539. (Biology). View Reference
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Knowles R. Reinherz E, Haynes B, Nadler L, Bernstein I, ed. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.
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Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986; 137(8):2501-2507. (Biology). View Reference
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Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
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van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.