Performance without peer, choice without compromise
The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.
Flexibility in a Small Space
An affordable choice to fit most flow cytometry analyzer needs, the BD LSRFortessa™ system can be configured with up to 7 lasers*—blue, red, violet, UV and yellow-green. This flexibility in laser wavelengths allows assay design to be optimized using the latest fluorescent dyes and substrates. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today’s assay requirements. The BD LSRFortessa is an innovative and proven platform for multicolor analysis.
The BD LSRFortessa puts the power of the proven BD™ LSR platform into a compact footprint. It can easily fit on the benchtop for cost effective space use.
This space efficiency is even more important for labs with limited space or where space cost is at a premium. In addition to the reduced size, design enhancements provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup.
Many innovations are incorporated into the BD LSRFortessa product line. The heart of the cytometer, the fluidics system features a true fixed alignment flow cell that is gel-coupled to the collection optics to maximize detector signal
The fluidics system is pressure driven. Hydrodynamic focusing forces sample cells through the cuvette flow cell, where they are interrogated. The flow cell is in fixed alignment with the laser and gel-coupled to the collection optics. This design ensures that the laser is precisely focused on the sample stream and the maximum amount of emitted light can be collected for added sensitivity in multicolor applications. Fixed alignment also minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.
The sheath container (8 L) and waste container (10 L) are outside the cytometer, and positioned on the floor for easier access.
The optional BD FACSFlow™ supply system fluidics cart increases capacity and ease of use while maintaining a stable fluidics pressure. It includes an automated sheath and waste fluid control system that reduces daily maintenance by incorporating two 20-L containers (Cubitainers®).
Fluidic sensors maintain constant pressure, while a fluidics monitoring system warns when sheath fluid is low, empty, or when the waste container is full.
Maximum Signal, Minimum Crosstalk
The patented collection optics are yet another design innovation. Arranged in octagon- and trigon-shaped optical pathways, their novel design efficiently maximizes signal detection and increases sensitivity and resolution. This allows researchers to identify cells, especially dim and rare cell populations, optimizing multicolor assays and panel design for superior results.
The optics system consists of laser excitation optics that illuminate cells in the sample, and collection optics that direct light scatter and fluorescence signals through spectral filters to detectors. Innovative designs for both the excitation optics and collection optics in BD LSRFortessa systems reduce excitation losses and optimize collection efficiency for increased sensitivity and resolution.
The excitation optics consist of multiple fixed wavelength lasers, beam shaping optics, and individual pinholes which result in spatially separated beam spots.
A final lens focuses the laser light into the gel-coupled cuvette flow cell. Since the optical pathway and the sample core stream are fixed, alignment is constant from day to day and from experiment to experiment.
Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The collection optics are set up in patented octagon- and trigon-shaped optical pathways that maximize signal detection resulting from each laser illuminated beam spot. Bandpass filters in front of each PMT allow spectral selection of the collected wavelengths. Importantly, this arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength.
Transmission pathways in an octagon
Emission spectra of commonly used fluorochromes
This design is based on the principle that light reflection is more efficient than light transmission. Emitted light travels to each PMT detector via reflection and is transmitted through only two pieces of glass to reach the detector. Therefore, more colors can be detected with minimum light loss.
BD FACSDiva™ software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS™ instrument families, including the BD FACSCanto™ cell analyzer and BD FACSAria™ cell sorter systems. This affords researchers greater application flexibility allowing them to easily move assays from one platform to another.
Cytometer Setup and Tracking
The Cytometer Setup and Tracking (CS&T) feature of BD FACSDiva software establishes baseline settings and adjusts for instrument variability. The software reduces operator error, and ensures consistency of results by setting the signal time delay across the multiple laser beams and optimizing PMT voltages. Application specific settings can also be set, allowing for rapid setup and performance of routine experiments in a more consistent manner. Quality control (QC) tracking capabilities in the software measure instrument settings and report on performance. Levey-Jennings plots help users understand instrument performance and identify maintenance issues.
Acquisition and Analysis
BD FACSDiva software enables researchers to preview and record data from multiple samples with an automated acquisition process. Acquisition templates, user-definable experiment layouts, and simple compensation procedures are also managed by the software to further facilitate acquisition.
BD FACSDiva worksheet showing well defined T-cell population subsets acquired using the special order BD LSRFortessa analyzer configured with the following lasers: 60-mW 355-nm UV, 100-mW 450-nm violet, 100-mW 488-nm blue, and 40-mW 640-nm red.
For analysis, the software includes powerful features such as hierarchical snap-to gating, a variety of plot formats, and batch analysis. Recorded data can be analyzed by creating plots, gates, population hierarchies, and statistical views on a BD FACSDiva global worksheet. Once the global worksheet is saved, it can be used to analyze multiple sample tubes from an experiment, thereby saving time. Numerous other productivity benefits include user-definable batch analysis and automated features such as gate resizing, pausing between data files, exporting statistics, and printing before proceeding to next data file.
BD™ High Throughput Sampler Option
To improve experimental workflow, the optional BD™ High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from 96- and 384-well microtiter plates. In high-throughput mode, the HTS option can speed through a 96-well plate in fewer than 15 minutes with less than 0.5% sample carryover from one well to the next. Low carryover is essential in research applications to ensure sample purity and data integrity.
Fast acquisition speed is achieved by synchronizing two high-precision pumps for sample mixing, sample injection, and probe washing. Standard throughput mode can be selected for acquisition of larger sample volumes.
Special Order Products
The BD special order research program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Tailored to the special needs of research at the leading edge of biomedical discovery, the program offers a wide range of choices to help researchers create the ultimate customized instrument for their requirements.
Contact your local BD Instrument Sales Representative to learn more about our special order cell analyzers.
BD LSRFortessa™ Violet Laser (405 nm)
- BD Horizon™ BV421 (ex 407 nm/em 421 nm)
- BD Horizon™ V450 (ex 404 nm/em 448 nm)
- Pacific Blue™ (ex 401 nm/em 452 nm)
- AmCyan (ex 457 nm/em 491 nm)
- BD Horizon™ V500 (ex 415 nm/em 500 nm)
- BD Horizon™ BV510 (ex 405 nm/em 510 nm)
- BD Horizon™ BV605 (ex 407 nm/em 602 nm)
- BD Horizon™ BV711 (ex 405 nm/em 711 nm)
BD LSRFortessa Blue Laser (488 nm)
- Alexa Fluor® 488 (ex 495 nm/em 519 nm)
- FITC (ex 494 nm/em 519 nm)
- PE (ex 496 nm/em 578 nm)
- BD Horizon™ PE-CF594 (ex 496 nm/em 612 nm)
- PE-Cy™5 (ex 496 nm/em 667 nm)
- PerCP (ex 482 nm/em 678 nm)
- PerCP-Cy™5.5 (ex 482 nm/em 695 nm)
- PE-Cy™7 (ex 496 nm/em 785 nm)
BD LSRFortessa Yellow-Green Laser (561 nm)
- PE (ex 564 nm/em 578 nm)
- BD Horizon™ PE-CF594 (ex 564 nm/em 612 nm)
- PE-Cy™5 (ex 564 nm/em 667 nm)
- PE-Cy™7 (ex 564 nm/em 785 nm)
BD LSRFortessa Red Laser (640 nm)
- An Introduction to Window Extension on Digital Flow Cytometers
- Construction of Multicolor Antibody Panels for the Flow Cytometric Analysis of Murine Thymic Stromal Cells
Product Information Sheets
Quick Reference Guides
- BD FACSDiva™ Software v8.0 Administrative Tasks
- BD FACSDiva™ Software v8.0 for BD LSRFortessa™ Flow Cytometers
- BD FACSDiva™ Software v8.0 for BD LSRFortessa™ Flow Cytometers (with HTS Option)
- BD FACSDiva™ Software v6.1 and Earlier
- BD™ High Throughput Sampler (HTS) Option for the BD LSRFortessa™ Flow Cytometer
- BD LSRFortessa™ Cell Analyzer
- Special Order BD LSRFortessa™ Cell Analyzer
User's Guides and Manuals
- Cross-Instrument and Cross-Site Standardization Using BD FACSDiva Software's Custom Application Settings: Part I of II
- Cross-Instrument and Cross-Site Standardization Using BD FACSDiva Software's Custom Application Settings: Part II of II
- Instrument Characterization and Performance Tracking for Digital Flow Cytometers
- The Digital Flow Cytometer: Performing Instrument Characterization for Optimal Setup
- Using the BD™ Cytometer Setup and Tracking (CS&T) System for Instrument Characterization and Performance Tracking
BD flow cytometers are Class I (1) laser products.
Alexa Fluor® and Texas Red® are registered trademarks of Molecular Probes, Inc.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited. Product availability and prices are subject to change without notice.
* To meet evolving application requirements, the BD LSRFortessa supports up to 4 lasers which can be upgraded to 7 lasers through the special order program, either at the time of purchase or at a later date.
Alexa Fluor® is a registered trademark and Pacific Blue™ is a trademark of Molecular Probes, Inc.
CF is a trademark of Biotium, Inc.
Cy™ is a trademark of Amersham Biosciences Corp. Cy™ dyes are subject to proprietary rights of Amersham Biosciences Corp and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from Amersham Biosciences Corp, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.