BD Pharmingen™ Purified Mouse Anti-Human RIP with Control
Clone G322-2 (RUO)
Western blot analysis of RIP. Lysate from Jurkat cells was probed with anti-RIP (clone G322-2, Comp. No. 51-6559GR) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). RIP is identified as a band of 74 kDa.
Western blot analysis of RIP. Lysate from Jurkat cells was probed with anti-RIP (clone G322-2, Comp. No. 51-6559GR) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). RIP is identified as a band of 74 kDa.
Description
RIP (receptor interacting protein) is a 74 kDa serine/threonine kinase which may be recruited to TNFR type 1 and Fas (CD95) receptor signal complexes following ligand binding. RIP interacts with other signal proteins within these complexes (e.g., RAIDD) and has also been shown to interact with pro-caspase-2. RIP contains an N-terminal kinase domain as well as a C-terminal death domain that is homologous to intracellular death domain of Fas. Over expression of RIP in vitro is sufficient to induce cell death, demonstrating that RIP functions as an apoptosis-inducing protein. Interaction of the Fas death domain with other intracellular proteins like RIP is an important step leading to downstream components in apoptotic signaling pathways.
Clone G322-2 recognizes human RIP. A recombinant truncated human RIP:tagged fusion protein, lacking the kinase domain of RIP, was used as immunogen. The specificity of the antibody was verified by ELISA, immunoprecipitation and western blot analysis. The antibody is routinely tested by western blot analysis in human Jurkat T cells where it recognizes RIP as a 74 kDa band. Smaller molecular weight breakdown bands of ~30 kDa, 22 kDa, and/or 16 kDa are sometimes observed.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml. Additional control lysate (Cat. No. 611451) is sold separately.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
| Description | Quantity/Size | Part Number | Clone | Isotype |
|---|---|---|---|---|
| Purified Mouse Anti-Human RIP | 50 µg (3 ea) | 51-6559GR | G322-2 | IgG1, |
| Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A | N/A |
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.