Apoptosis Protocols

Sample Preparation

Induction FAS

Receptor-Mediated Apoptosis in Mouse Cells Using anti-Fas/CD95 (Clone Jo2)

Find the procedure for evaluating induction of apoptosis in mouse cells using anti-mouse Fas monoclonal antibody, clone Jo2.

Receptor-Mediated Apoptosis in Human Cells Using anti-Fas/CD95 (Clone DX2)

Find a general ELISA protocol and helpful hints for detecting and quantitating cytokines.

Cell Death

Induction of Apoptosis by Treatment with Staurosporine

Get the protocol for inducing apoptosis using the non-selective protein kinase inhibitor staurosporine.

Induction of Apoptosis by Treatment with Camptothecin

Find the protocol for inducing apoptosis using camptothecin, the potent inhibitor of topoisomerase I.

Annexin V Staining Protocol

Get the step-by-step protocol for annexin V staining and blocking, along with suggestions for controls for flow cytometric analysis of annexin V samples.

APO-BRDU™ Procedure

Explore protocols for fixation of cells, staining and for measuring apoptosis in the positive and negative control cells and cell specimens.

APO-DIRECT™ Procedure

Explore the staining protocol for flow cytometry analysis of apoptosis using the BD Pharmingen™ Apo-Direct™ Kit.