Preparation And Storage
Recommended Assay Procedures
ELISA (IL-12 p70 Detection): The Biotin Mouse Anti-Human IL-12 (p40/p70) (Clone C8.6, Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p70). Biotinylated C8.6 antibody can be paired with the Purified Rat Anti-Human IL-12 p70 mAb (Clone 20C2, Cat. No. 555065) as the capture antibody and recombinant human IL-12 (p70) protein (Cat. No. 554613) as the standard. For testing human IL-12 (p70) in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IL-12 (p70) ELISA Set (Cat. No. 555183).
ELISA (IL-12 p40 Detection): The Biotin Mouse Anti-Human IL-12 (p40/p70) (Clone C8.6, Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p40). Biotinylated C8.6 antibody can be paired with the Purified Mouse Anti-Human IL-12 (p40) mAb (Clone C8.3, Cat. No. 551227) as the capture antibody with recombinant human IL-12 (p40) protein (Cat. No. 554633) as the standard. For testing human IL-12 (p40) in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IL-12 (p40) ELISA Set (Cat. No. 555171).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The C8.6 monoclonal antibody specifically recognizes human IL-12 p40 that is encoded by IL12B (interleukin 12B). The antibody binds to IL-12 p40 monomers and homodimers as well as to the p40 subunit of the heterodimeric cytokines, IL-12 p70 (p35/p40) and IL-23 (p19/p40). These cytokines are produced by a variety of cells, including activated monocytes, macrophages, neutrophils, and dendritic cells. IL-12 p40 monomers and homodimers can block the binding of heterodimeric IL-12 to its receptor. IL-12 promotes the generation of Th1 cells and activates NK cells, Group 1 innate lymphoid cells (ILC1), and cytotoxic T cells leading to production of IFN-γ, TNF, granzymes, and perforin. IL-23 promotes the generation and activation of Th17 cells and other effector cells, including γδ T cells, NKT cells, MAIT cells, and ILC3 that can produce cytokines including IL-17A, IL-17F, IL-22, GM-CSF, TNF, and IL-6. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and can reportedly inhibit the biological activities of IL-12 and IL-23.
Development References (3)
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D'Andrea A, Aste-Amezaga M, Valiante NM, Ma X, Kubin M, Trinchieri G. Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells. J Exp Med. 1993; 178(3):1041-1048. (Clone-specific: Neutralization). View Reference
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D'Andrea A, Rengaraju M, Valiante NM, et al. Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J Exp Med. 1992; 176(5):1387-1398. (Clone-specific: Neutralization). View Reference
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Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.. Immunity. 2000; 13(5):715-25. (Clone-specific: Immunoprecipitation). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.