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BD Pharmingen™ Purified Rat Anti-Mouse CD19
Clone 1D3 (RUO)
Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 (Cat. No. 553517) and either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified rat anti-mouse CD19 mAb 1D3 (right panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig (Cat. No. 554016). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 (Cat. No. 553517) and either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified rat anti-mouse CD19 mAb 1D3 (right panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig (Cat. No. 554016). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 (Cat. No. 553517) and either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified rat anti-mouse CD19 mAb 1D3 (right panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig (Cat. No. 554016). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were simultaneously stained with PE-conjugated mouse anti-mouse IgM[a] mAb DS-1 (Cat. No. 553517) and either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified rat anti-mouse CD19 mAb 1D3 (right panel). The staining of primary antibody was detected with FITC-conjugated goat anti-rat Ig (Cat. No. 554016). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Product Details
BD Pharmingen™
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Mouse CD19 Transfected Cell Line
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Functional assay, Immunoprecipitation (Reported)
0.5 mg/ml
AB_395047
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Purified Rat IgG2a, κ Isotype Control RUO
Size
0.5 mg
Cat No.
553927
FITC Goat Anti-Rat Ig RUO
Size
0.5 mg
Cat No.
554016
553783 Rev. 17
Antibody Details
1D3
The 1D3 antibody reacts with CD19, a B lymphocyte-lineage differentiation antigen. CD19, a 95-kDa transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. Terminally differentiated plasma cells do not express CD19. On the surface of mature B cells, the CD19 molecule associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with CD19-deficient mice have suggested that the level of CD19 expression affects the generation and maturation of B cells in the bone marrow and periphery. B-1 lineage B cells, also known as CD5+ B cells, are drastically reduced or absent in CD19-deficient mice. Increased levels of CD19 expression correlate with increased frequencies of peritonal and splenic B-1 cells and reduced numbers of conventional B lymphocytes in the periphery. CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
553783 Rev. 17
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553783 Rev.17
Citations & References
View product citations for antibody "553783" on CiteAb
Development References (12)
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Engel P, Zhou LJ, Ord DC, Sato S, Koller B, Tedder TF. Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule. Immunity. 1995; 3(1):39-50. (Biology). View Reference
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Fearon DT. The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes. Curr Opin Immunol. 1993; 5(3):341-348. (Biology). View Reference
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Gommerman JL, Oh DY, Zhou X, et al. A role for CD21/CD35 and CD19 in responses to acute septic peritonitis: a potential mechanism for mast cell activation. J Immunol. 2000; 165(12):6915-6921. (Biology). View Reference
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Inaoki M, Sato S, Weintraub BC, Goodnow CC, Tedder TF. CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes. J Exp Med. 1997; 186(11):1923-1931. (Biology). View Reference
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Krop I, Shaffer AL, Fearon DT, Schlissel MS. The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996; 157(1):48-56. (Biology: Functional assay). View Reference
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Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996; 26(1):238-242. (Immunogen: Functional assay, Immunoprecipitation). View Reference
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Rickert RC, Rajewsky K, Roes J. Impairment of T-cell-dependent B-cell responses and B-1 cell development in CD19-deficient mice. Nature. 1995; 376(6538):352-355. (Biology). View Reference
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Sato S, Jansen PJ, Tedder TF. CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling. Proc Natl Acad Sci U S A. 1997; 94(24):13158-13162. (Biology). View Reference
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Sato S, Miller AS, Howard MC, Tedder TF. Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19. J Immunol. 1997; 159(7):3278-3287. (Biology). View Reference
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Sato S, Ono N, Steeber DA, Pisetsky DS, Tedder TF. CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity. J Immunol. 1996; 157(10):4371-4378. (Biology). View Reference
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Sato S, Steeber DA,Jansen PJ, Tedder TF. CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19. J Immunol. 1997; 158(10):4662-4669. (Biology). View Reference
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Tedder TF, Zhou LJ, Engel P. The CD19/CD21 signal transduction complex of B lymphocytes. Immunol Today. 1994; 15(9):437-442. (Biology). View Reference
553783 Rev. 17
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.