Fluorochrome Faceoff

Watch your favourite fluorochromes take on the competition!

For Professionals in Research

Welcome to the BD Biosciences Fluorochrome Faceoff, where we explore the performance of various flow cytometry dyes excited primarily by the blue and yellow-green lasers! Follow along with this series of “games” where we highlight key fluorochrome attributes to keep in mind when designing flow cytometry panels and review performance data to shine a light on the best-performing fluorochromes within each category.

The first game results post on Tuesday, September 10, 2024. Stay tuned over the three weeks following to see how your favourite fluorochromes stand up to the competition.

Follow #FluorochromeFaceoff on LinkedIn for game updates.

Results and conclusions shown throughout the Fluorochrome Faceoff are based on experiments performed under the conditions described. Users should evaluate reagents with their specific protocols as results may vary with different experimental conditions.

Fluorochrome Family Summary

To view the games, click on the links in the first row of the table below

Dye Family	Spillover	Resolution	Buffer Compatibility	Intracellular Staining	Photostability	Monocyte Background
What Good Looks Like:	1–2 Peak/s	3–4 Relative Brightness	Perm III; >50% MFI Compared to Control after Treatment	Catalog Availability	<25% MFI Loss vs Time 0	No Nonspecific Binding
BD Horizon RealYellow™ and RealBlue™ Reagents
StarBright™ Blue and Yellow Reagents
NovaFluor™ Blue and Yellow Reagents
BD Horizon Brilliant™ Blue Reagents
PE and PE Tandems
PerCP and PerCP Tandems