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For Professionals in Research


The BD® ELISPOT Assay is a powerful tool for detecting and analyzing individual cells that secrete a particular protein in vitro. BD® ELISPOT Reagents coupled with a high-throughput ELISPOT plate reader provide intuitive analysis of cytokines at the single-cell level.


The reagents:

  •  Provide direct analysis of frequency of cells producing cytokines and other effector molecules using an enzyme-linked immunospot (ELISPOT) assay 
  • Offer greater sensitivity than ELISAs and intracellular staining
  • Enable high-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells
  • Offer the flexibility to choose among several formats: 
    • BD® ELISPOT Kits—ready-to-use kits with precoated BD® ELISPOT Plates for human, nonhuman primate and mouse research
    • BD® ELISPOT Reagent Sets—coat-it-yourself sets with sufficient capture antibody, detection antibody and streptavidin-horseradish peroxidase for 10 plates worth of assays (includes 10 uncoated plates)
    • BD® ELISPOT Reagent Pairs–matched antibody pairs. Sufficient capture antibody and detection antibody for 5 plates

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.