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      PE Mouse Anti-Rat IgM
      33634907
      EA (1 Each)
      0.2 mg
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      Product Details
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse IgG1, κ
      Pooled Rat Ig
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_395117
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      PE-conjugated G53-238 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC-conjugated G53-238 mAb (Cat. No. 533887).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      Data Sheets

      DownloadTechnical Data Sheet (TDS)DownloadSafety Data Sheet
      553888 Rev. 9
      Antibody Details
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      G53-238

      The G53-238 antibody reacts specifically with rat IgM monomers and pentamers. It does not react with other Ig isotypes. G53-238 antibody has not been shown to stimulate B-cell proliferation.

      553888 Rev. 9
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      496 nm, 566 nm
      576 nm
      553888 Rev.9
      Citations & References
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      No Citations Found

      No Citations Are Available for this Product

      553888 Rev. 9

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.