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Product Details
BD Pharmingen™
Mouse BALB/c IgG1, κ
Flow cytometry, Isotype control (Routinely Tested)
0.2 mg/ml
AB_10050483
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry).
We recommend PE-conjugated mouse IgG1 κ mAb MOPC-21 (Cat. No. 555749) for immunofluorescent staining of human whole blood, mAb MOPC-21 (Cat. No. 554680 or 559320) for intracellular cytokine flow cytometry, and mAb MOPC-21 (Cat. No. 556650) for non-human primate cells.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
PE Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555749
PE Mouse IgG1, κ Isotype Control RUO
Size
50 Tests
Cat No.
556650
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
PE Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
559320
550617 Rev. 5
Antibody Details
MOPC-31C
The MOPC-31C antibody has unknown specificity. The transplantable plasmacytoma MOPC-31C was induced by intraperitoneal injection of mineral oils into BALB/c mice. It was adapted to continuous cell culture by alternate passage in animals.
550617 Rev. 5
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
550617 Rev.5
Citations & References
View product citations for antibody "550617" on CiteAb
Development References (2)
-
Hay R, Caputo J, Chen TR, Macy M, McClintock P, Reid Y, ed. ATCC. Cell Lines and Hybridomas, Eighth Edition. 1994:75.
-
Sibinovic KH, Potter M, Hoostelaere, Rode B, Wax J, ed. Catalogue of plasmacytomas and other tumors of the lymphoreticular system, 3rd edition. Kensington, Maryland: Litton Bionetics, Inc; 1976:1-33.
550617 Rev. 5
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.