Uncover 30 Human Immune Markers in a Single Experiment
The BD® OMICS-One Immune Profiler Protein Panel (BD® OMICS-One IP Protein Panel) is developed using our antibody-oligo based technology and consists of 30 different specificities against most major human immune markers lyophilized in a single tube.
Designed and optimized to work on the BD Rhapsody™ Single-Cell Analysis System, the BD® OMICS-One IP Protein Panel is tested to work seamlessly with BD Rhapsody™ Single-Cell RNA Assays and BD® Single-Cell Multiplexing Kits to enable CITE-seq studies.
Find more information in the BD® OMICS-One Immune Profiler Protein Panel brochure.
List of BD® OMICS-One Immune Profiler Protein Panel Specificities
30 pre-titrated antibodies
| Specificity | Clone |
|---|---|
| CD3 | UCHT1 |
| CD4 | SK3 |
| CD8 | SK1 |
| CD11c | B-Ly6 |
| CD14 | MΦP9 |
| CD16 | 3G8 |
| CD19 | SJ25C1 |
| CD25 | 2A3 |
| CD27 | M-T271 |
| CD28 | L293 |
| Specificity | Clone |
|---|---|
| CD45RA | HI100 |
| CD56 | NCAM16 |
| CD62L | DREG-56 |
| CD127 | HIL-7R-M21 |
| CD134 | ACT35 |
| CD137 | 4B4-1 |
| CD161 | HP-3G10 |
| CD183 (CXCR3) | 1C6/CXCR3 |
| CD185 (CXCR5) | RF8B2 |
| CD186 (CXCR6) | 13B 1E5 |
| Specificity | Clone |
|---|---|
| CD196 (CCR6) | 11A9 |
| CD197 (CCR7) | 2-L1-A |
| CD272 | J168-540 |
| CD278 | DX29 |
| CD279 | EH12.1 |
| CD357 (GITR) | V27-580 |
| CD366 (TIM-3) | 7D3 |
| HLA-DR | G46-6 |
| IgD | IA6-2 |
| IgM | G20-127 |
Performance
Similar performance between the BD® OMICS-One IP Protein Panel versus freshly mixed BD® AbSeq Antibodies. Following isolation from whole blood, peripheral blood mononuclear cells (PBMC) were split into resting (untreated) and activated (treated with CD3/CD28 for 24 hours) groups. A 1:1 mixture of the resting and activated cells were stained with either the BD® OMICS-One IP Protein Panel or a freshly prepared mixture of the same AbSeq specificities. Equal number of cells were then loaded onto the BD Rhapsody™ Cartridges and AbSeq and WTA libraries were generated and sequenced (n = 2 individual experiments for this study). Data were analyzed using SeqGeq™ Software.
A. UMAP demonstrated strong overlap in the cell groups identified between the BD® OMICS-One IP Protein Panel and fresh BD® AbSeq Antibody-stained samples.
B. The total number of AbSeq molecules detected by the BD® OMICS-One IP Protein Panel and fresh BD® AbSeq Antibody mixture showed a high correlation with R2 greater than 0.98.
Performance of all 30 AbSeq specificities included in the BD® OMICS-One IP Protein Panel. PBMCs were activated and prepared as described in the figure above. After staining, cells were captured on the BD Rhapsody™ System and AbSeq and WTA libraries were generated and sequenced. To obtain over 80% sequencing saturation, the libraries were sequenced at 20,000 reads/cell for WTA and 30,000 reads/cell for AbSeq using the Illumina™ NextSeq™ High-Output Kit. Data were analyzed using SeqGeq™ Software. We repeated the above experiments with at least two different donors. The representative figures from one donor are shown here.
A. Cell annotation of UMAP of resting + activated PBMCs resolved by the BD® OMICS-One IP Protein Panel antibodies and the WTA mRNA profile.
B. Heat maps of each AbSeq clone from BD® OMICS-One IP Protein Panel on UMAP from (A) showing the specificity of AbSeq detection for individual cell type.
The BD® OMICS-One IP Protein Panel is a flexible backbone panel and accommodates additional AbSeq specificities. Three BD® AbSeq Antibodies were added and mixed with the reconstituted BD® OMICS-One IP Protein Panel pellet (n = 2).
A. The BD® OMICS-One IP Protein Panel performance was not impacted by drop-ins as shown by high correlation (R2 over 0.99 with or without drop-ins).
B. The BD® OMICS-One IP Protein Panel specificities of CD8 (SK1) and CD45RA (top row) were assessed against the specificity of drop-ins CD8 [RPA-T8], CD45RO and CD38 (bottom row) and are shown in UMAP. Drop-in for CD38 detected cell types that are expected to be positive for CD38. Drop-in clone for CD8 (RPA-T8) showed a staining pattern very similar to the BD® OMICS-One IP Protein Panel clone (SK1) suggesting the high specificity of drop-in antibody as well as the compatibility of two clones for the same antigen. The contrasting expression pattern of CD45RO (drop-in) compared to CD45RA (BD® OMICS-One IP Protein Panel) further confirmed that adding the AbSeq specificities to the BD® OMICS-One IP Protein Panel had no adverse impact on experimental outcomes.
The BD® OMICS-One IP Protein Panel is designed to work with RNA and multiplexing assays
A. WTA and AbSeq libraries from BD® OMICS-One IP Protein Panel-stained cells (1:1 mixture of resting and activated PBMCs) were generated and sequenced. To illustrate the power of multiomic analysis, we analyzed the WTA data only (mRNA analysis) and compared with WTA + AbSeq data (mRNA and protein analysis). UMAP coordinates and unbiased clustering (Phenograph) using only WTA (mRNA) data are shown on the left, while coordinates and annotations using WTA + AbSeq (mRNA and protein) data are shown on the right. With a multiomics approach, additional cell types were revealed offering deeper biological insights.
B. To test the compatibility of the BD® Single-Cell Multiplexing Kit (SMK) and the BD® OMICS-One IP Protein Panel, we performed cell staining with the SMK and the BD® OMICS-One IP Protein Panel together and generated WTA, AbSeq and SMK libraries for sequencing. The expression of markers in the BD® OMICS-One IP Protein Panel was then compared to data generated in the absence of the SMK. These data showed that addition of the SMK does not impact the BD® OMICS-One IP Protein Panel as demonstrated by high correlation (R2 >0.99) between the BD® OMICS-One IP Protein Panel + WTA versus BD® OMICS-One IP Protein Panel + WTA + SMK.
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Brochure
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For Research Use Only. Not for use in diagnostic or therapeutic procedures. Refer to Manufacturer's instructions for use and related user manuals and technical sheets before using this product as described.