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PE Rat Anti-Mouse Vβ4 T-Cell Receptor
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PE Rat Anti-Mouse Vβ4 T-Cell Receptor
Two-color analysis of the expression of Vβ4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with PE-conjugated KT4, FITC-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553046/553047), and FITC-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
Two-color analysis of the expression of Vβ4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with PE-conjugated KT4, FITC-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553046/553047), and FITC-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
KT4 B10.D2 mouse T-cell clone I3
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394812
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553366 Rev. 11
Antibody Details
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KT4

The KT4 antibody specifically recognizes the Vβ4 T-cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells.

553366 Rev. 11
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
553366 Rev.11
Citations & References
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View product citations for antibody "553366" on CiteAb

Development References (3)

  1. Katz JD, Benoist C, Mathis D. T helper cell subsets in insulin-dependent diabetes. Science. 1995; 268(5214):1185-1188. (Biology). View Reference
  2. Sugihara S, Fujiwara H, Shearer GM. Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions. J Immunol. 1993; 150(2):683-694. (Biology). View Reference
  3. Tomonari K, Lovering E, Spencer S. Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype. Immunogenetics. 1990; 31(5-6):333-339. (Immunogen). View Reference
553366 Rev. 11

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.