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PE Mouse Anti-Human Folate Receptor β (FRβ)
PE Mouse Anti-Human Folate Receptor β (FRβ)

Two-parameter flow cytometric analysis of Folate Receptor β (FR-β) expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 54680; Left Plot) or PE Rat Anti-Human Folate Receptor β (FRβ) antibody (Cat. No. 567708; Right Plot). After staining, erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A bivariate pseudocolor density plot showing the correlated expression of Folate Receptor β (FRβ)  [or Ig Isotype control staining] versus side light-scatter signals was derived from gated events with the light scattering characteristics for viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

Two-parameter flow cytometric analysis of Folate Receptor β (FR-β) expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 54680; Left Plot) or PE Rat Anti-Human Folate Receptor β (FRβ) antibody (Cat. No. 567708; Right Plot). After staining, erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A bivariate pseudocolor density plot showing the correlated expression of Folate Receptor β (FRβ)  [or Ig Isotype control staining] versus side light-scatter signals was derived from gated events with the light scattering characteristics for viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

Product Details
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BD Pharmingen™
FOLR2; folate receptor beta; Folate Receptor β; FRbeta; FR-BETA; FR-β; FRβ
Human (QC Testing)
Mouse BALB/c IgG1, κ
Mouse FRβ Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  7. An isotype control should be used at the same concentration as the antibody of interest.
567708 Rev. 1
Antibody Details
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94b

The 94b monoclonal antibody specifically recognizes the human Folate Receptor beta (Folate Receptor β or FRβ). This receptor is a glycosylphosphatidylinositol (GPI)-linked membrane protein that is encoded by FOLR2 which belongs to the folate-binding receptor family. These cell surface receptors share ~70% amino acid sequence homology and bind folate with high affinity. The Folate Receptor β (FRβ) transports folate into cells which is essential for methionine and DNA synthesis that support cell growth and proliferation. FRβ is expressed on placental cells and on monocytes as well as macrophages found in normal and diseased tissues, eg, in rheumatoid arthritis and tumors. Soluble forms of FRβ have also been described which can be found in various body fluids such as plasma or milk.

567708 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
567708 Rev.1
Citations & References
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Development References (5)

  1. Holm J, Hansen SI. Characterization of soluble folate receptors (folate binding proteins) in humans. Biological roles and clinical potentials in infection and malignancy. Biochim Biophys Acta Proteins Proteom. 2020; 1868(10):1-10. (Biology). View Reference
  2. McNulty H, Pentieva K, Hoey L, Strain J, Ward M. Nutrition throughout life: folate.. Int J Vitam Nutr Res. 2012; 82(5):348-54. (Biology). View Reference
  3. Nagai T, Furusho Y, Li H, et al. Production of a High-affinity Monoclonal Antibody Reactive with Folate Receptors Alpha and Beta.. Monoclon Antib Immunodiagn Immunother. 2015; 34(3):181-90. (Clone-specific: Flow cytometry). View Reference
  4. Nagayoshi R, Nagai T, Matsushita K, et al. Effectiveness of anti-folate receptor beta antibody conjugated with truncated Pseudomonas exotoxin in the targeting of rheumatoid arthritis synovial macrophages. Arthritis Rheum. 2005; 9(9):2666-2675. (Immunogen). View Reference
  5. Otsubo H, Tsuneyoshi Y, Nakamura T, Matsuda T, Komiya S, Matsuyama T. Serum-soluble folate receptor β as a biomarker for the activity of rheumatoid arthritis synovitis and the response to anti-TNF agents.. Clin Rheumatol. 2018; 37(11):2939-2945. (Clone-specific). View Reference
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567708 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.