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BV480 Mouse Anti-Human CD36
Product Details
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BD OptiBuild™
GPIIIb; Glycoprotein IIIb; PAS IV; Platelet glycoprotein IV; Scavenger receptor class B, member 3; Thrombospondin receptor
Human (Tested in Development)
Mouse IgG1, κ
Fetal Erythrocytes
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  8. Researchers should determine the optimal concentration of this reagent for their individual applications.
  9. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
756786 Rev. 1
Antibody Details
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FA6-152

The FA6-152 monoclonal antibody specifically binds to CD36, an ~88 kDa transmembrane glycoprotein which belongs to the class B scavenger receptor family. This scavenger receptor contains a heavily glycosylated extracellular domain followed by two transmembrane segments and N- and C-terminal cytoplasmic tails. CD36 is expressed on platelets, megakaryocytes, monocytes/macrophages, dendritic cells (DCs), erythroid precursors, microvascular endothelia, skeletal, cardiac, and smooth muscle cells, adipocytes, and epithelia of retina, breast, and intestine. This multifunctional receptor plays essential roles in lipid homeostasis, angiogenesis, immune response, adhesion, and cancer progression. CD36 is also a very early marker of erythroid differentiation. In platelets, CD36 promotes activation, aggregation and secretion. Among blood cells, the FA6-152 antibody binds to both adult and fetal monocytes, platelets, and reticulocytes, but does not react with lymphocytes and granulocytes. This antibody reportedly blocks CD36 interactions with thrombospondin, collagen, apoptotic cells, and modified LDL and induces agglutination of fetal but not adult erythrocytes.

756786 Rev. 1
Format Details
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BV480
The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 will also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV480
Violet 405 nm
440 nm
479 nm
756786 Rev.1
Citations & References
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Development References (7)

  1. Edelman P, Vinci G, Villeval JL, et al. A monoclonal antibody against an erythrocyte ontogenic antigen identifies fetal and adult erythroid progenitors.. Blood. 1986; 67(1):56-63. (Immunogen: Fluorescence activated cell sorting). View Reference
  2. Handunnetti SM, van Schravendijk MR, Hasler T, Barnwell JW, Greenwalt DE, Howard RJ. Involvement of CD36 on erythrocytes as a rosetting receptor for Plasmodium falciparum-infected erythrocytes.. Blood. 1992; 80(8):2097-104. (Biology). View Reference
  3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  4. Silverstein RL, Febbraio M. CD36, a scavenger receptor involved in immunity, metabolism, angiogenesis, and behavior.. Sci Signal. 2009; 2(72):re3. (Biology). View Reference
  5. Tandon NN, Kralisz U, Jamieson GA. Identification of glycoprotein IV (CD36) as a primary receptor for platelet-collagen adhesion.. J Biol Chem. 1989; 264(13):7576-83. (Biology). View Reference
  6. Zola H. CD36. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:97. View Reference
  7. de Haas M, von dem Borne AEG. CD36 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:636-637.
View All (7) View Less
756786 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.