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555026Image1.png

Flow cytometric analysis of CD31 expression on rat splenic leukocytes. LEW splenocytes were stained with FITC Mouse Anti-Rat CD45RA (Cat. No. 554883) and Biotin Mouse Anti-Rat CD31 (Cat. No. 555026; Right Panel), followed by PE Streptavidin (Cat. No. 554061). Contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ system.

22712D_555026_image2.png

Flow cytometric analysis of CD31 expression on rat splenic leukocytes. LEW splenocytes were stained with FITC Mouse Anti-Rat CD45RA (Cat. No. 554883) and Biotin Mouse Anti-Rat CD31 (Cat. No. 555026; Right Panel), followed by PE Streptavidin (Cat. No. 554061). Contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ system.

555026Image1.png 22712D_555026_image2.png Blue-Cap-White-Label.jpg clear-clear-white.jpg

Flow cytometric analysis of CD31 expression on rat splenic leukocytes. LEW splenocytes were stained with FITC Mouse Anti-Rat CD45RA (Cat. No. 554883) and Biotin Mouse Anti-Rat CD31 (Cat. No. 555026; Right Panel), followed by PE Streptavidin (Cat. No. 554061). Contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ system.

Flow cytometric analysis of CD31 expression on rat splenic leukocytes. LEW splenocytes were stained with FITC Mouse Anti-Rat CD45RA (Cat. No. 554883) and Biotin Mouse Anti-Rat CD31 (Cat. No. 555026; Right Panel), followed by PE Streptavidin (Cat. No. 554061). Contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ system.

Product Details
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BD Pharmingen™
PECAM-1; Pecam1; Pecam
Rat (QC Testing), Pig (Tested in Development)
Mouse BALB/c IgG1, κ
Lewis Rat Microglia
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_395656
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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555026 Rev. 6
Antibody Details
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TLD-3A12

The TLD-3A12 antibody specifically recognizes CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed on endothelial cells, platelets, and subsets of leucocytes. In the human and mouse, multiple alternatively spliced isoforms have been identified, and this alternative splicing may be involved in the regulation of ligand specificity. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis in the mouse and rat. In addition, PECAM-1 has been demonstrated to play an important role in extravasation of leucocytes in vivo, but in vivo treatment with TLD-3A12 mAb had no discernable effect upon the inflammatory infiltrate in experimental allergic encephalomyelitis. The TLD-3A12 mAb partially blocks the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This mouse anti-rat CD31 antibody crossreacts with pig endothelial cells and platelets, as determined by immunohistochemical staining of acetone-fixed frozen sections and immunofluorescent staining with flow cytometric analysis, respectively. A suspension of Lewis rat microglial cells was used as the the immunogen.

555026 Rev. 6
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
555026 Rev.6
Citations & References
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View product citations for antibody "555026" on CiteAb

Development References (5)

  1. DeLisser HM, Christofidou-Solomidou M, Strieter RM, et al. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol. 1997; 151(3):671-677. (Biology). View Reference
  2. DeLisser HM, Newman PJ, Albelda SM. Molecular and functional aspects of PECAM-1/CD31. Immunol Today. 1994; 15(10):490-495. (Biology). View Reference
  3. Famiglietti J, Sun J, DeLisser HM, Albelda SM. Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity. J Cell Biol. 1997; 138(6):1425-1435. (Biology). View Reference
  4. Wakelin MW, Sanz MJ, Dewar A, et al. An anti-platelet-endothelial cell adhesion molecule-1 antibody inhibits leukocyte extravasation from mesenteric microvessels in vivo by blocking the passage through the basement membrane. J Exp Med. 1996; 184(1):229-239. (Biology). View Reference
  5. Williams KC, Zhao RW, Ueno K, Hickey WF. PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J Neurosci Res. 1996; 45(6):747-757. (Immunogen). View Reference
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555026 Rev. 6

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.