Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
New Zealand (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      Alexa Fluor® 647 Mouse Anti-Human Hobit (ZNF683)
      Alexa Fluor® 647 Mouse Anti-Human Hobit (ZNF683)
      Multicolor flow cytometric analysis of Hobit (ZNF683) expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD3 (Cat. No. 562426/562427), PE Mouse Anti-Human CD56 (Cat. No. 563238), and Alexa Fluor® 647 Mouse Anti-Human Hobit (ZNF683) (Cat. No. 566250) antibodies. Two-color flow cytometric contour plots showing the correlated expression of CD3 versus Hobit (ZNF683) (Left Plot) or CD56 versus Hobit (ZNF683) (Right Plot) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      Alexa Fluor® 647 Mouse Anti-Human Hobit (ZNF683)
      Multicolor flow cytometric analysis of Hobit (ZNF683) expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD3 (Cat. No. 562426/562427), PE Mouse Anti-Human CD56 (Cat. No. 563238), and Alexa Fluor® 647 Mouse Anti-Human Hobit (ZNF683) (Cat. No. 566250) antibodies. Two-color flow cytometric contour plots showing the correlated expression of CD3 versus Hobit (ZNF683) (Left Plot) or CD56 versus Hobit (ZNF683) (Right Plot) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      HOBIT; Homolog of Blimp-1 in T cells; Zinc finger protein 683; ZNF683
      Human (QC Testing)
      Mouse BALB/c IgM, κ
      Human Hobit Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      AB_2739629
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      566250 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      Sanquin-Hobit/1

      The Sanquin-Hobit/1 monoclonal antibody specifically binds to Homolog of Blimp-1 in T cells (Hobit), which is also known as Zinc finger protein 683 (ZNF683). Hobit is a conserved Zn-finger transcription factor that is homologous to Blimp-1. It is expressed in cytotoxic effector-type CD8+ T cells and in some effector memory CD8+ T cells, but is not significantly expressed in naïve or most central memory CD8+ T cells. Hobit is also expressed in natural killer (NK) cells, and NKT cells, but is not expressed in CD4+ T cells, B cells, monocytes, dendritic cells, or neutrophils. Hobit expression is strongly correlated with the expression T-bet and Interferon-γ (IFN-γ) mRNA. Hobit appears to play a role in the differentiation and effector functions of NK cells and effector CD8+ T cells such as in the production of IFN-γ.

              

      566250 Rev. 1
      Format Details
      Down Arrow Up Arrow
      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      566250 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "566250" on CiteAb

      Development References (3)

      1. Braun J, Frentsch M, Thiel A. Hobit and human effector T-cell differentiation: The beginning of a long journey.. Eur J Immunol. 2015; 45(10):2762-5. (Biology). View Reference
      2. Vieira Braga FA, Hertoghs KM, Kragten NA, et al. Blimp-1 homolog Hobit identifies effector-type lymphocytes in humans.. Eur J Immunol. 2015; 45(10):2945-58. (Immunogen: Flow cytometry). View Reference
      3. van Gisbergen KP, Kragten NA, Hertoghs KM, et al. Mouse Hobit is a homolog of the transcriptional repressor Blimp-1 that regulates NKT cell effector differentiation.. Nat Immunol. 2012; 13(9):864-71. (Biology). View Reference
      566250 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.