PE Mouse Anti-Human bcl-2

BD™ PE Mouse Anti-Human bcl-2

Name: PE Mouse Anti-Human bcl-2
Brand: BD™
SKU: 340576

Clone Bcl-2/100

(RUO)

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Product Details

Brand: BD™

Alternative Name: BCL2; Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Bcl-2 synthetic peptide aa. 41-54

Application: Flow cytometry

Concentration: 1 μg/mL

Vol. Per Test: 20 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The FITC and PE conjugates are each supplied as 1 μg in 1.0 mL (1 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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Antibody Details

Bcl-2/100

The Anti–bcl-2 antibody, clone 100, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with the bcl-2α peptide (amino acids 41-54).

The Anti–bcl-2 antibody recognizes a 26-kilodalton (kDa) protein encoded by the 230 kb bcl-2 protooncogene. The protein is a regulator of the apoptotic process, and elevated levels can provide resistance to cell death. The bcl-2 protein is expressed in the interior of cells, frequently localized to the mitochondrial membrane.

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Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

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Citations & References

Development References (11)

Aiello A, Delia D, Borrello MG, et al. Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells. Cytometry. 1992; 13(5):502-509. (Biology). View Reference
Campos L, Rouault J-P, Sabido O, et al. High expression of bcl-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy. Blood. 1993; 81:3091-3096. (Biology).
Cleary ML, Smith SD, Sklar J. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 1986; 47:19-28. (Biology).
Coustan-Smith E, Kitanaka A, Pui C-H, et al. Clinical relevance of bcl-2 overexpression in childhood acute lymphoblastic leukemia. Blood. 1996; 87:1140-1146. (Biology).
DiGiuseppe JA, LeBeau P, Augenbraun J, Borowitz MJ. Multiparameter flow-cytometric analysis of bcl-2 and fas expression in normal and neoplastic hematopoiesis. Am J Clin Pathol. 1996; 106:345-351. (Biology).
Hermine O, Haioun C, Lepage E, et al. Prognostic significance of bcl-2 protein expression in aggressive non-Hodgkin's lymphoma. Blood. 1996; 87:265-272. (Biology).
Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W, Reed JC. Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res. 1993; 53(19):4701-4714. (Biology). View Reference
Pezzella F, Tse AG, Cordell JL, Pulford KA, Gatter KC, Mason DY. Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol. 1990; 137(2):225-232. (Biology). View Reference
Reed JC. Double identity for proteins of the bcl-2 family. Nature. 1997; 387:773-776. (Biology).
Tsujimoto Y, Croce CM. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci U S A. 1986; 83(14):5214-5218. (Biology). View Reference
Zamai L, Canonico B, Gritzapis A, et al. Intracellular detection of Bcl-2 and p53 proteins by flow cytometry: comparison of monoclonal antibodies and sample preparation protocols. J Biol Regul Homeost Agents. 2002; 16:289–302. (Biology).

View All (11)

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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.