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FITC Mouse Anti-Human TCR-α/β
Product Details
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αβ TCR; TCR alpha-beta
Mouse IgG1, κ
Human Thymocytes
Flow cytometry
50 μg/mL
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation And Storage

The monoclonal antibody is supplied as 12.5 µg purified immunoglobulin in 2.0 mL (6.25 µg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 100 µg in 2.0 mL (50 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347773 Rev. 1
Antibody Details
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Clone WT31 is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

WT31 (Anti–TCR-α/β-1) recognizes a conformational epitope formed by the T-cell receptor (TCR) for antigen and the CD3 epsilon chain. The α/β TCR is a disulfide-linked 80-kDalton (kDa) heterodimer consisting of a 44-kDa α chain and a 37-kDa β chain.

347773 Rev. 1
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
347773 Rev.1
Citations & References
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Development References (9)

  1. Brenner M, McClean J, Dialynas D, et al. Identification of a putative second T cell receptor. Nature. 1986; 322:145. (Biology).
  2. Gupta S, Shimizu M, Ohira K, Vayuvegula B. T cell activation via the T cell receptor: a comparison between WT31 (defining alpha/beta TcR)-induced and anti-CD3-induced activation of human T lymphocytes.. Cell Immunol. 1991; 132(1):26-44. (Biology). View Reference
  3. Lanier LL, Weiss A. Presence of Ti (WT31) negative T lymphocytes in peripheral blood and thymus. Nature. 1986; 324:268. (Biology).
  4. Oettgen HC, Kappler J, Tax WJ, Terhorst C. Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J Biol Chem. 1984; 259(19):12039-12048. (Biology). View Reference
  5. Slameron A, Sanchez-Madrid F, Ursa MA, Fresno M, and Alarcon B. A conformational epitope expressed upon association of the CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 1991; 147:3047-3052. (Biology).
  6. Spits H, Borst J, Tax W, Capel PJ, Terhorst C, de Vries JE. Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen.. J Immunol. 1985; 135(3):1922-8. (Biology). View Reference
  7. Tax WJ, Leeuwenberg HF, Willems HM, Capel PJ, Koene RA. Monoclonal antibodies reactive with OKT3 antigen of OKT8 antigen. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies : specification, classification, nomenclature = Typage leucocytaire : antigènes de différenciation leucocytaire humains révélés par les anticorps monoclonaux : "Rapports des études communes". Berlin New York: Springer-Verlag; 1984:721-722.
  8. Tax WJ, Willems HW, Reekers PP, Capel PJ, Koene RA. Polymorphism in mitogenic effect of IgG1 monoclonal antibodies against T3 antigen on human T cells.. Nature. 304(5925):445-7. (Biology). View Reference
  9. Weiss A, Newton M, Crommie D. Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer.. Proc Natl Acad Sci USA. 1986; 83(18):6998-7002. (Biology). View Reference
View All (9) View Less
347773 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.