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APC Mouse Anti-Human CD11c
Product Details
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BD™
ITGAX; Integrin alpha-X; AlphaX integrin chain; CR4; Leu M5; p150/95 alpha
Human
Mouse IgG2b
Flow cytometry
5 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO


Preparation And Storage

The PE conjugate is supplied as 25 μg in 2.0 mL (12.5 μg/mL). The APC conjugate is supplied in 0.5 mL. Please refer to the vial label for APC antibody concentration. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340544 Rev. 1
Antibody Details
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SHCL-3

The CD11c antibody, clone S-HCL-3, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from CD-1 mice immunized with whole cells and membrane preparations of hairy-cell leukemia.

The CD11c antibody recognizes a human monocyte/macrophage antigen. CD11c is specific for the 150-kilodalton (kDa) α-subunit of the CD11c/CD18 antigen heterodimer, also referred to as complement receptor type 4 (CR4). The 95-kDa CD18 antigen, the β chain of leucocyte function–associated antigen-1 (LFA-1), is common to the CD11a/CD18 (LFA-1) and CD11b/CD18 antigen heterodimers.

340544 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC
Red 627-640 nm
651 nm
660 nm
340544 Rev.1
Citations & References
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Development References (9)

  1. Grouard G, Durand I, Filgueira L, Banchereau J, Liu YJ. Dendritic cells capable of stimulating T cells in germinal centres.. Nature. 1996; 384(6607):364-7. (Biology). View Reference
  2. Kurzinger K, Ho M-K, Springer TA. Structural homology of a macrophage differentiation antigen and an antigen involved in T-cell mediated killing. Nature. 1982; 290:688. (Biology).
  3. Lanier LL, Arnaout MA, Schwarting R, Warner NL, Ross GD. p150/95, Third member of the LFA-1/CR3 polypeptide family identified by anti-Leu M5 monoclonal antibody. Eur J Immunol. 1985; 15(7):713-718. (Biology). View Reference
  4. Myones BL, Dalzell JG, Hogg N, Ross GD. Neutrophil and monocyte cell surface p150,95 has iC3b-receptor (CR4) activity resembling CR3.. J Clin Invest. 1988; 82(2):640-51. (Biology). View Reference
  5. O'Doherty U, Peng M, Gezelter S, et al. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature.. Immunology. 1994; 82(3):487-93. (Biology). View Reference
  6. Olweus J, BitMansour A, Warnke R, et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci USA. 1997; 94:12551-12556. (Biology).
  7. Sanchez-Madrid F, Simon P, Thompson S, Springer TA. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983; 158(2):586-602. (Biology). View Reference
  8. Schwarting R, Stein H, Wang CY. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985; 65(4):974-983. (Biology). View Reference
  9. Thomas R, Lipsky PE. Human peripheral blood dendritic cell subsets. Isolation and characterization of precursor and mature antigen-presenting cells.. J Immunol. 1994; 153(9):4016-28. (Biology). View Reference
View All (9) View Less
340544 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.