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TNFRSF7; Tumor necrosis factor receptor superfamily, member 7; Tp55; S152
Mouse BALB/c IgG1
Human Activated Peripheral Blood Cells
Flow cytometry
6 μg/mL
5 μL
VI T6T037

655019 Rev. 1
Antibody Details
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The CD27 antibody, clone L128, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with activated peripheral blood lymphocytes.

The CD27 antibody recognizes a 110–120-kilodalton (kDa) disulfide-linked homodimer comprised of two 55-kDa polypeptide chains. The CD27 antigen is a lymphocyte-specific member of the tumor necrosis factor receptor (TNFR) super family, which also includes nerve growth factor receptor, CD30, CD40, CD95 (Fas), CD120a, CD120b, CD134 (OX 40), and CD137. The CD27 antigen is also known as S152, T14, TNFRSF7, and Tp55.

655019 Rev. 1
Format Details
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Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Red 627-640 nm
651 nm
660 nm
655019 Rev.1
Citations & References
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View product citations for antibody "655019" on CiteAb

Development References (21)

  1. Akiba H, Nakano H, Nishinaka S, et al. CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-κB and stress-activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-κB-inducing kinase. J Biol Chem. 1998; 273:13353-13358. (Biology).
  2. Bowman MR, Crimmins MA, Yetz-Aldape J, Kriz R, Kelleher K, Herrmann S. The cloning of CD70 and its identification as the ligand for CD27. J Immunol. 1994; 152(4):1756-1761. (Biology). View Reference
  3. Camerini D, Walz G, Loenen WA, Borst J, Seed B. The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family. J Immunol. 1991; 147(9):3165-3169. (Biology). View Reference
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Gruss HJ, Dower SK. Tumor necrosis factor ligand superfamily: involvement in the pathology of malignant lymphomas. Blood. 1995; 85:3378-3404. (Biology).
  7. He L, Fox MH. Comparison of flow cytometry and western blotting to measure Hsp70. Cytometry. 1996; 25:280-286. (Biology).
  8. Hintzen RQ, De Jong R, Lens SM, Brouwer M, Baars P, Van Lier RA. Regulation of CD27 expression on subsets of mature T lymphocytes. J Immunol. 1993; 151:2426-2435. (Biology).
  9. Hintzen RQ, Lens SM, Beckmann MP, Goodwin RG, Lynch D, van Lier RA. Characterization of the human CD27 ligand, a novel member of the TNF gene family. J Immunol. 1994; 152(4):1762-1773. (Biology). View Reference
  10. Hintzen RQ, de Jong R, Hack CE, et al. A soluble form of the human T cell differentiation antigen CD27 is released after triggering of the TCR/CD3 complex. J Immunol. 1991; 147(1):29-35. (Biology). View Reference
  11. Kobata T, Agematsu K, Kameoka J, Schlossman SF, Morimoto C. CD27 is a signal-transducing molecule involved in CD45RA+ naive T cell costimulation. J Immunol. 1994; 153(12):5422-5432. (Biology). View Reference
  12. Kobata T, Jacquot S, Kozlowski S, Agematsu K, Schlossman SF, Morimoto C. CD27-CD70 interactions regulate B-cell activation by T cells. Proc Natl Acad Sci U S A. 1995; 92:11249-11253. (Biology).
  13. Kobata T, Morimoto C. CD27 Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:67-69.
  14. Maurer D, Fischer G, Fae I, et al. IgM and IgG but not cytokine secretion is restricted to the CD27+ Blymphocyte subset. J Immunol. 1992; 148:3700-3705. (Biology).
  15. Reiter C. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:341-342.
  16. Reiter C. T9. Cluster report: CD27. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:350.
  17. Sugita K, Dasgupta J, Mojima Y, Agematsu K, Schlossman S, Morimoto C. Protein kinase A-mediated phosphorylation of the T-cell surface antigen CD27. Immunology. 1993; 80:217-221. (Biology).
  18. Sugita K, Robertson MJ, Torimoto Y, Ritz J, Schlossman SF, Morimoto C. Participation of the CD27 antigen in the regulation of IL-2-activated human natural killer cells. J Immunol. 1992; 149:1199-1203. (Biology).
  19. Watts TH. TNF/TNFR family members in costimulation of T cell responses. Annu Rev Immunol. 2005; 23:23-68. (Biology). View Reference
  20. Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
  21. de Jong R, Loenen WA, Brouwer M, et al. Regulation of expression of CD27, a T cell-specific member of a novel family of membrane receptors. J Immunol. 1991; 146:2488-2494. (Biology).
View All (21) View Less
655019 Rev. 1

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Analyte Specific Reagent. Analytical and performance characteristics are not established.

Analyte Specific Reagent. Analytical and performance characteristics are not established.


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