BD Horizon™ Red 718 Research Reagents
Innovative fluorochrome designed to provide greater resolving power than Alexa Fluor™ 700
BD Horizon™ Red 718 Reagents
BD Horizon™ Red 718 (R718) is an innovative small-molecule fluorochrome developed exclusively for BD Biosciences. It provides improved sensitivity and resolution of low-density markers and intracellular targets. R718 is excited by the red laser with an emission maximum of 718 nm and can be detected by the same filter as Alexa Fluor™ 700.
BD Horizon™ Red 718 Reagents are specially designed to serve as alternatives to Alexa Fluor™ 700 or APC-R700.
BD Horizon™ Red 718 Reagents offer:
- Greater brightness and resolving power than Alexa Fluor™ 700 for both surface and intracellular markers
- Reduced background staining compared to Alexa Fluor™ 700 and APC-R700
- Minimal spread into the APC channel
- A broad portfolio of conjugates through BD OptiBuild™ On-Demand Reagents
BD Horizon™ Red 718 Reagents have the potential to enhance your flow cytometry panel's performance.
BD Horizon™ Red 718 Reagents
Two-color comparison staining of IL-17A on stimulated human peripheral blood lymphocytes.
Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate and Ionomycin in the presence of BD GolgiStop™ Protein Transport Inhibitor. The cells were permeabilized with BD Perm/Wash™ Buffer then stained with BD Horizon™ R718 Mouse Anti-Human IL-17A (clone N49-653, A) or BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human IL-17A (clone N49-653, B) and BD Pharmingen™ PE Mouse Anti-Human CD4 (clone RPA-T4) shown as two-color flow cytometric pseudocolor density plots derived from gated lymphocytes and were analyzed the same day on a BD LSRFortessa™ X-20 Cell Analyzer with FlowJo™ Software.
Comparison staining of CD4 on human lysed whole blood. A. Lysed whole blood stained with BD Horizon™ R718 Mouse Anti-Human CD4 (clone SK3, red) or BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human CD4 (clone SK3, grey) shown overlaid as fluorescence histograms derived from a lymphocyte gate. B. SSC vs. fluorescence plots of BD Horizon™ R718 Mouse Anti-Human CD4 (left) or BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human CD4 (right). All data were analyzed on a BD LSRFortessa™ X-20 Cell Analyzer with FlowJo™ Software.
A. BD Horizon™ R718 FoxP3 is on par with the gold standard BD Pharmingen™ PE FoxP3. Human PBMCs were stained with BD Horizon™ R718 Mouse Anti-Human FoxP3 (clone 259D/C7).
B. BD Phosflow™ Treated Human Control Cells (red) and BD Phosflow™ Untreated Human Control Cells (grey) were stained with BD Phosflow™ R718 Mouse Anti-Stat5 (pY694) (clone 47); treated and untreated control cell overlays shown here are derived from a CD4+ gate.
BD OptiBuild™ On-Demand R718 Reagents Offer Superior Resolution
Comparison of BD Horizon™ R718 Reagents (red), BD OptiBuild™ On-Demand Red 718 Reagents equivalent (blue), vs. BD Pharmingen™ Alexa Fluor™ 700 (orange) using surface markers mouse anti-human CD3 (left, clone UCHT1) or rat anti-mouse CD8a (right, clone 53-6.7)
|Reagent Specificity||Species||Clone||Isotype||Size||Catalog Number|
|BCL-6||Human||K112-91||Mouse IgG1, κ||100 Tests||566979|
|CD3||Human||UCHT1||Mouse IgG1, κ||100 Tests||566953|
|CD4||Human||SK3||Mouse IgG1, κ||100 Tests||566930|
|CD11c||Human||B-ly6||Mouse IgG1, κ||100 Tests||566932|
|CD19||Human||SJ25C1||Mouse IgG1, κ||100 Tests||566946|
|Human||EH12.1||Mouse IgG1, κ||100 Tests||566974|
|FoxP3||Human||259D/C7||Ms IgG1||100 Tests||566935|
|IL-17A||Human||N49-653||Mouse IgG1, κ||100 Tests||566938|
|Mouse IgG1, κ||100 Tests||566977|
|CD107a||Mouse||1D4B||Rat IgG2a, κ||50 ug||566986|
|CD4||Mouse||RM4-5||Rat IgG2a, k||50 ug||566939|
|CD8a||Mouse||53-6.7||Rat IgG2a, κ||50 ug||566985|
|CD13||Mouse||R3-242||Rat IgG1||50 ug||566982|
|Ly-6C||Mouse||AL-21||Rat IgG2a, κ||50 ug||566987|
Alexa Fluor® 700 is a trademark of Thermo Fisher Scientific.
Class 1 Laser Product. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
BD, the BD Logo, Cytofix/Cytoperm, FlowJo, GolgiStop, Horizon, LSRFortessa, OptiBuid, Pharmingen and Phosflow are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners. © 2020 BD. All rights reserved.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.