BD Horizon™ Real Fluorochromes

Interview with Jochen Lamote, Head of VIB Flow Core

BD Biosciences sat down with Jochen Lamote to discuss his experience as a beta tester of flow cytometry reagents – including how BD Horizon RealBlue^™ minimizes spillover and simplifies panel design.

Watch the interview to discover why the team at Vlaams Institute for Biotechnology (VIB) Flow Core would recommend BD Horizon RealBlue^™to their colleagues.

Minimize Cross-laser Spillover. Optimize Panel Resolution.

Format	Spectral	Conventional	Spillover (1-low, 4-high)	Alternative To	Products
Violet Laser
RV828	✓	‒	1	Qdot^™ 800, NovaFluor^™ V800	View
Blue Laser
RB545	✓	‒	1	(Unique position)	View
RB613	✓	✓	2	PE-CF594, PE-Dazzle^™ 594, BB630-P2, StarBright^™ Blue 615	View
RB670	✓	✓	2	PE-Cy5, BB660-P2, StarBright^™ Blue 675	View
RB705	✓	✓	2	PerCP-Cy5.5, BB700, StarBright^™ Blue 700	View
RB744	✓	✓	1	BB755-P	View
RB780	✓	✓	1	PE-Cy7, BB790-P	View
RB824	✓	✓	1	PerCP/Fire^™806, StarBright^™Blue 810	View
Yellow-Green Laser
RY586	✓	✓	1	PE	View
RY610	✓	✓	1	PE-CF594, PE-Dazzle^™ 594	View
RY655	✓	✓	3	PE-Cy5, StarBright Yellow 665	View
RY703	✓	✓	2	PE-Cy5.5, StarBright^™ Yellow 720	View
RY743	✓	✓	2	PE/Fire^™ 744	View
RY775	✓	✓	2	PE-Cy7, StarBright^™ Yellow 800	View
Red Laser
RR688	✓	‒	1	Alexa Fluor^™ 660, Spark NIR^™685, NovaFluor^™ R685, cFluor^™ R685	View

“The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

Dr. Florian Ebner

Distinguished Lab Scientist, Cancer Pharmacology and Disease Positioning

Boehringer Ingelheim RCV GmbH & Co KG

“I’m really excited about the BD Horizon RealBlue^™ 780 Dye. The staining we have done so far with Ki-67 and Granzyme B can be seen very clearly, even with high dilutions.”

Johannes Brandi

Researcher

Bernhard Nocht Institute for Tropical Medicine

“CXCR5 in Real Blue 780 has great spectral characteristics and complements perfectly our panel for spectral cytometry. Due to its brightness, RB 780 can be used with low concentrations. In general, the contact to BD representatives is effortless and easy.”

Jakov Korzhenevich

President - Young Scientist Association

Immunology, Medical University of Vienna

"BD’s RealDye-conjugated antibodies allowed me to overcome the problems from working with high autofluorescent cells. The bright and well-defined peaks helped me getting a clean spectral unmixing and expand the number of markers resolved with spectral flow cytometry. Adding RB744 to our panels even became an internal joke – you add it because “it is such a nice color”

Luís Crisóstomo

PhD, Senior Researcher

University of Turku, Finland

RESOURCES

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Brochures

BD Horizon RealBlue™ 545 Reagents

BD Horizon RealBlue™ 613 and BD Horizon RealYellow ™ 610

BD Horizon RealBlue™ 705 Reagents

BD Horizon RealBlue™ 744 Reagents

BD Horizon RealBlue™ 780 Reagents

BD Horizon RealYellow™ 586 Reagents

Performance Guides

BD Fluorochrome Performance Guide

Terms and Conditions

Offer valid from January 1st 2025 – March 31st 2025. Cannot be combined with any other offer. Valid in participating countries only. Valid once per user. Offer valid on BD Horizon RealBlue™ 670 (RB670) Reagents 25 test/ug size only. There are a limited number of samples available, and BD retains the right to cancel this offer at any time.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD, the BD Logo, BD Horizon RealBlue and BD Horizon RealYellow are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners. © 2024 BD. All rights reserved. BD-132104 (v1.0) 0724 CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

BD FACSLyric^™ Flow Cytometer with the BD FACSuite^™Clinical and BD FACSuite^™ applications is an in vitro diagnostic medical device bearing a CE mark.