Purified Mouse Anti- PCMT-I/II

BD Transduction Laboratories™ Purified Mouse Anti- PCMT-I/II

Name: Purified Mouse Anti- PCMT-I/II
Brand: Purified Mouse Anti- PCMT-I/II
SKU: 610772

Clone 4/PCMT-II (RUO)

Western blot analysis of PCMT-I/II on a A431 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- PCMT-I/II antibody.

Immunofluorescence staining of human intestinal smooth muscle cells (HISM).

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Catalog No:610772

List Price:Inquire

Unit:EA (1 Each)

Size:

50 µg

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Product Details

Brand: BD Transduction Laboratories™

Alternative Name: Protein Carboxyl Methyl Transferase; PIMT

Reactivity: Human (QC Testing), Dog (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Human PCMT-II aa. 10-211

Application: Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)

Target Molecular Weight: 25 kDa

Concentration: 250 µg/ml

RRID: AB_398093

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Data Sheets

Technical Data Sheet (TDS)

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610772 Rev. 1

Antibody Details

4/PCMT-II

PCMT-II (Protein Carboxyl Methyl Transferase), or L-isoaspartate (D-aspartate) O-methyltransferase, is a cytosolic enzyme that is expressed in all tissues as well as in bacteria. Interestingly, PCMT-II is highly expressed in the brain. It recognizes damaged proteins that result from deamidation, isomerization, and racemization reactions. These proteins contain L-isoaspartyl and D-aspartyl residues and can have abnormal configurations that result in anomalous or loss of function. PCMT-II catalyzes the first step of the conversion of these unusual residues to normal L-aspartyl residues. This mechanism prevents the accumulation of aberrant proteins in aging cells. Pcmt-1 knock-out mice display growth retardation and a seizure disorder that results in death at an average age of 42 days. Thus, PCMT-II is necessary for growth and nervous system function. The immunogen region from human PCMT-II used to generate this antibody has been reported to be identical to the region in PCMT-I.

610772 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

610772 Rev.1

Citations & References

View Development References

Development References (5)

Galus A, Lagos A, Romanik EA, O'Connor CM. Structural analysis of transcripts for the protein L-isoaspartyl methyltransferase reveals multiple transcription initiation sites and a distinct pattern of expression in mouse testis: identification of a 5'-flanking sequence with promoter activity. Arch Biochem Biophys. 1994; 312(2):524-533. (Biology). View Reference
Kim E, Lowenson JD, MacLaren DC, Clarke S, Young SG. Deficiency of a protein-repair enzyme results in the accumulation of altered proteins, retardation of growth, and fatal seizures in mice. Proc Natl Acad Sci U S A. 1997; 94(12):6132-6137. (Biology). View Reference
MacLaren DC, Clarke S. Expression and purification of a human recombinant methyltransferase that repairs damaged proteins. Protein Expr Purif. 1995; 6(1):99-108. (Biology). View Reference
MacLaren DC, Kagan RM, Clarke S. Alternative splicing of the human isoaspartyl protein carboxyl methyltransferase RNA leads to the generation of a C-terminal -RDEL sequence in isozyme II. Biochem Biophys Res Commun. 1992; 185(1):277-283. (Biology). View Reference
Romanik EA, Ladino CA, Killoy LC, D'Ardenne SC, O'Connor CM. Genomic organization and tissue expression of the murine gene encoding the protein beta-aspartate methyltransferase. Gene. 1992; 118(2):217-222. (Biology). View Reference

View All (5)

610772 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.