Description
Interleukin-1 (IL-1β) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Mouse IL-1 is a 17 kD protein containing 153 amino acid residues.
Formulation and Purity: Recombinant mouse IL-1β is supplied lyophilized from a solution comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.4, and containing 50 µg bovine serum albumin, per µg of cytokine. The product contains no preservatives. Recombinant mouse Interleukin-1β is > 95% pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.
Preparation And Storage
The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5-10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
Recommended Assay Procedures
1. Biological Activity: As measured by proliferation of TF-1 indicator cells at 10^5 cells/ml
Specific Activity: 0.06 - 2.5 x10^8 Unit*/mg
ED50: 0.04-1.5 ng/ml; Observed dose response relationship: >50 fold
*Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Investigators are advised to determine optimal concentrations for individual applications.
2. ELISA Standard:.
Recombinant mouse IL-1β is useful as a quantitative standard for measuring mouse IL-1β protein levels in an IL-1β -specific sandwich ELISA. For specific methodology, please visit the online protocols under "ELISA and ELISPOT" at our website, http://www.bdbiosciences.com/us/s/resources. To measure mouse IL-1β in plasma or serum, we recommend the BD OptEIA™ Mouse IL-1β ELISA Set (Cat. No. 559603), which is specially formulated for assay of cytokine in complex fluids.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Development References (4)
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Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). View Reference
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Gray PW, Glaister D, Chen E, Goeddel DV, Pennica D.. Two interleukin 1 genes in the mouse: cloning and expression of the cDNA for murine interleukin 1 beta.. J Immunol. 1986; 137(11):3644-3648. (Biology).
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Kitamura T, Takaku F, Miyajima A. IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. Int Immunol. 1991; 3(6):571-577. (Biology). View Reference
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Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Methodology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.