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Product Details
BD Pharmingen™
Cdh5; Cadherin-5; CADH5; VE-cadherin; Vascular endothelial cadherin; 7B4
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Mouse VE-Cadherin-Ig Fusion
Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
0.5 mg/ml
None
AB_395707
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555289 Rev. 6
Antibody Details
11D4.1
The 11D4.1 antibody monoclonal antibody specifically binds to mouse CD144, also known as VE-cadherin. CD144 is a type I transmembrane protein and is a member of the cadherin superfamily. VE-cadherin is an endothelial cell-specific, homophilic adhesion molecule. It is concentrated at interendothelial cells contacts and is thought to be involved in the maintenance of cell layer integrity. In vitro and in vivo studies indicate that the 11D.4 mAb interferes with VE-cadherin-mediated intercellular adhesion.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
555289 Rev. 6
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555289 Rev.6
Citations & References
View product citations for antibody "555289" on CiteAb
Development References (3)
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Breier G, Breviario F, Caveda L, et al. Molecular cloning and expression of murine vascular endothelial-cadherin in early stage development of cardiovascular system.. Blood. 1996; 87(2):630-41. (Biology: Immunohistochemistry). View Reference
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Gotsch U, Borges E, Bosse R, et al. VE-cadherin antibody accelerates neutrophil recruitment in vivo. J Cell Sci. 1997; 110(5):583-588. (Immunogen: Blocking, Immunoprecipitation). View Reference
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Lampugnani MG, Resnati M, Raiteri M, et al. A novel endothelial-specific membrane protein is a marker of cell-cell contacts.. J Cell Biol. 1992; 118(6):1511-22. (Biology). View Reference
555289 Rev. 6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.