Flow cytometric analysis of mouse Ig, κ light chain expression on mouse splenocytes. Splenocytes from BALB/c mice were stained with PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with a BD Horizon™ V450 Rat IgG1, κ Isotype Control (Cat. No. 560535, Left Panel) or with the BD Horizon™ V450 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561354, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Flow cytometric analysis of mouse Ig, κ light chain expression on mouse splenocytes. Splenocytes from BALB/c mice were stained with PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with a BD Horizon™ V450 Rat IgG1, κ Isotype Control (Cat. No. 560535, Left Panel) or with the BD Horizon™ V450 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561354, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Product Details
BD Horizon™
Ig kappa chain C region; IGKC; Igk-C; Ig, κ
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Flow cytometry (Routinely Tested)
0.2 mg/ml
16071
AB_10896989
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
V450 Rat IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
560535
PE Rat Anti-Mouse CD45R/B220 RUO
Size
0.2 mg
Cat No.
553090
.png?imwidth=320)
PE Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553089
561354 Rev. 1
Antibody Details
187.1
The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
561354 Rev. 1
Format Details
V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
V450
Violet 405 nm
405 nm
450 nm
561354 Rev.1
Citations & References
Development References (1)
-
Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference
561354 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
