Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
The 172224 monoclonal antibody specifically recognizes CD300 antigen-like family member A (CD300a) which is also known as Myeloid-associated immunoglobulin-like receptor 1 (MAIR-1 or MAIR-I), Mast cell-derived paired immunoglobulin-like receptor 1 (Mcpir1), CMRF35-like molecule 8 (CLM-8 or Clm8), Leukocyte mono-Ig-like receptor 1 (Lmir1), or Inhibitory receptor protein 60 (IRp60). CD300a is a single-pass type I membrane protein that is encoded by the Cd300a gene which belongs to the immunoglobulin gene superfamily. The extracellular region of CD300a contains an IgV-type domain whereas its intracellular region has three ITIMs and a non-canonical ITIM. CD300a is expressed on some B cells, NK cells, macrophages, dendritic cells, neutrophils, eosinophils, and mast cells. CD300a functions as an inhibitory receptor that can downregulate the effector functions of the cells it is expressed on.
Development References (3)
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Bachelet I, Munitz A, Mankutad D, Levi-Schaffer F. Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process.. J Biol Chem. 2006; 281(37):27190-6. (Biology). View Reference
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Carnec X, Meertens L, Dejarnac O, et al. The Phosphatidylserine and Phosphatidylethanolamine Receptor CD300a Binds Dengue Virus and Enhances Infection. J Virol. 2015; 90(1):92-102. (Clone-specific: Flow cytometry). View Reference
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Kumagai H, Oki T, Tamitsu K, et al. Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells.. Biochem Biophys Res Commun. 2003; 307(3):719-29. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.