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Product Details
BD Pharmingen™
Ighm; Igh-M; Immunoglobulin M; Igh6; muH; immunoglobulin heavy constant mu
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Pooled Mouse Ig
ELISA, Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394842
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures
For the sandwich mouse, Biotin Rat Anti-Mouse IgM (Cat. No. 553406) is optimal for detection with Purified Rat Anti-Mouse IgM (Cat. No. 553435) for capture. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC Rat Anti-Mouse IgM (Cat. No. 553437).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Purified Rat IgG2a, κ Isotype Control RUO
Size
0.5 mg
Cat No.
553927
FITC Goat Anti-Rat Ig RUO
Size
0.5 mg
Cat No.
554016
Biotin Rat Anti-Mouse IgM RUO
Size
0.5 mg
Cat No.
553406
Purified Rat Anti-Mouse IgM RUO
Size
0.5 mg
Cat No.
553435
553405 Rev. 13
Antibody Details
R6-60.2
The R6-60.2 antibody monoclonal antibody specifically binds to mouse Immunoglobulin M (IgM) of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. The R6-60.2 antibody has not been shown to stimulate B-cell proliferation.
553405 Rev. 13
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553405 Rev.13
Citations & References
View product citations for antibody "553405" on CiteAb
Development References (3)
-
BD Biosciences Pharmingen. Unpublished results. .
-
Gavin AL, Duong B, Skog P, et al. δBAFF, a splice isoform of BAFF, opposes full-length BAFF activity in vivo in transgenic mouse models. J Immunol. 2005; 75(1):319-328. (Clone-specific). View Reference
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Touma M, Keskin DB, Shiroki F, et al. Impaired B cell development and function in the absence of IκBNS. J Immunol. 2011; 187(8):3942-3952. (Clone-specific). View Reference
553405 Rev. 13
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.