Purified NA/LE Rat Anti-Mouse IL-3

BD Pharmingen™ Purified NA/LE Rat Anti-Mouse IL-3

Name: Purified NA/LE Rat Anti-Mouse IL-3
Brand: BD Pharmingen™
SKU: 554379

Clone MP2-8F8

(RUO)

Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG1

Immunogen: Recombinant mouse IL-3

Application: ELISA (Routinely Tested), Flow cytometry, Neutralization (Tested During Development)

Concentration: 1.0 mg/ml

RRID: AB_395356

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Recommended Assay Procedures

ELISA: The purified MP2-8F8 antibody (Cat. No. 554381) is useful as a capture antibody for a sandwich ELISA for measuring mouse IL-3 protein levels. Purified MP2-8F8 antibody can be paired with the biotinylated MP2-43D11 antibody (Cat. No. 554384) as the detecting antibody, with recombinant mouse IL-3 (Cat. No. 554579) as the standard. For testing mouse IL-3 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Mouse IL-3 ELISA Set (Cat. No. 555228).

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified NA/LE Rat IgG1 κ Isotype Control RUO

Size 0.5 mg Cat No. 553921

Mouse IL-3 ELISA Set RUO

Size 20 Plate(s) Cat No. 555228

Recombinant Mouse IL-3 RUO

Size 10 µg Cat No. 554579

554379 Rev. 3

Antibody Details

MP2-8F8

The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The immunogen used to generate the MP2-8F8 hybridoma was COS-expressed recombinant mouse IL-3.

554379 Rev. 3

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

554379 Rev.3

Citations & References

Development References (4)

Abrams JS, Pearce MK.. Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. J Immunol. 1988; 140(1):131-137. (Biology). View Reference
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology). View Reference
Cockayne DA, Abrams JS, Nienhuis AW. Antisense RNA inhibition of hematopoietic growth factor production. Growth Factors. 1991; 5(3):171-181. (Biology). View Reference
Weinstein Y, Ihle JN, Lavu S, Reddy EP. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc Natl Acad Sci U S A. 1986; 83(14):5010-5014. (Biology). View Reference

View All (4)

554379 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.