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      Purified NA/LE Rat Anti-Mouse CD132
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      BD Pharmingen™
      Il2rg; IL-2Rγ; Commin gamma chain; Common γ; gamma c receptor; γc
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG2a, κ
      Flow cytometry (Routinely Tested), Neutralization (Tested During Development)
      1.0 mg/ml
      AB_395402
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554454 Rev. 2
      Antibody Details
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      4G3

      The 4G3 monoclonal antibody specifically binds to the common γ subunit (γc) shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptors also known as CD132. This receptor is expressed constitutively at low levels on most lymphocytes, myeloid cells, embryonic thymocytes and lymphoid cell lines, but not non-lymphoid cells.  The γc receptor is a 75 - 80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated.

      554454 Rev. 2
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554454 Rev.2
      Citations & References
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      View product citations for antibody "554454" on CiteAb

      Development References (7)

      1. Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Biology). View Reference
      2. He YW, Adkins B, Furse RK, Malek TR. Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor. J Immunol. 1995; 154(4):1596-1605. (Biology). View Reference
      3. He YW, Malek TR. The IL-2 receptor gamma c chain does not function as a subunit shared by the IL-4 and IL-13 receptors. Implication for the structure of the IL-4 receptor. J Immunol. 1995; 155(1):9-12. (Biology). View Reference
      4. Kondo M, Takeshita T, Higuchi M, et al. Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes. Science. 1994; 263(5152):1453-1454. (Biology). View Reference
      5. Kondo M, Takeshita T, Ishii N, et al. Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4. Science. 1993; 262(5141):1874-1877. (Biology). View Reference
      6. Malek TR, Furse RK, Fleming ML, Fadell AJ, He YW. Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits. J Interferon Cytokine Res. 1995; 15(5):447-454. (Biology). View Reference
      7. Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
      View All (7) View Less
      554454 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.