Purified NA/LE Mouse Anti-Rat CD18

BD Pharmingen™ Purified NA/LE Mouse Anti-Rat CD18

Name: Purified NA/LE Mouse Anti-Rat CD18
Brand: Purified NA/LE Mouse Anti-Rat CD18
SKU: 554976

Clone WT.3 (RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Integrin β2 chain; integrin beta-2; Itgb2

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43

Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed, Immunoprecipitation (Reported)

Concentration: 1.0 mg/ml

RRID: AB_2128476

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified NA/LE Mouse IgG1 κ Isotype Control RUO

Size 0.5 mg Cat No. 553447

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

554976 Rev. 10

Antibody Details

WT.3

The WT.3 monoclonal antibody specifically recognizes the 95-100 kDa β2 subunit (CD18), which is found on the majority of leukocytes as a heterodimer with any of the three distinct CD11 α integrin subunits (CD11a or αL, CD11b or αM, CD11c or αX) to form, respectively, LFA-1, Mac-1, and gp150, 95. The function-blocking activity of WT.3 antibody has been determined in several in vitro assays measuring the binding of LFA-1 (αLβ2 integrin) to ICAM-1 (CD54). It has also been reported that WT.3 mAb inhibits leukocyte infiltration in an in vivo system.

554976 Rev. 10

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is =0.01 EU/µg (=0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

554976 Rev.10

Citations & References

View product citations for antibody "554976" on CiteAb

Development References (2)

Tamatani T, Kotani M, Miyasaka M. Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies. Eur J Immunol. 1991; 21(3):627-633. (Immunogen: Blocking, Immunoprecipitation). View Reference
Yamazaki T, Seko Y, Tamatani T, et al. Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules. Am J Pathol. 1993; 143(2):410-418. (Clone-specific: Blocking, Immunohistochemistry). View Reference

554976 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.