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Purified Mouse Anti-Human Wiskott-Aldrich Syndrome Protein
Purified Mouse Anti-Human Wiskott-Aldrich Syndrome Protein
Western Blot analysis of WASP in human histiocytic lymphoma. Lysate from U937 cells (ATCC CRL-1593.2) was probed with Mouse anti-Human WASP monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3).  WASP is identified as a band of 60 kDa.
Western Blot analysis of WASP in human histiocytic lymphoma. Lysate from U937 cells (ATCC CRL-1593.2) was probed with Mouse anti-Human WASP monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3).  WASP is identified as a band of 60 kDa.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human WASP Recombinant Protein
Western blot (Routinely Tested), Fluorescence microscopy, Immunoprecipitation, Intracellular staining (flow cytometry) (Reported)
60 kDa
0.25 mg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557773 Rev. 5
Antibody Details
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Wiskott-Aldrich syndrome (WAS) is an X-linked recessive immunodeficiency disease caused by mutations in the gene encoding WAS protein (WASP).  The disease is characterized by a spectrum of clinical signs, including thrombocytopenia, eczema, susceptibility to opportunistic and pyogenic infections, and B-cell lymphomas associated with Epstein-Barr virus.  Furthermore, patients' blood cells display morphological abnormalities that can be associated with an impaired cytoskeleton.  WASP is a member of a family of highly conserved proteins that link signaling pathways to the actin cytoskeleton.  These members include WASP, N-WASP (neuronal), and SCAR/WAVE isoforms (Suppressor of cAMP Receptor/WASP family Verprolin-homologous protein) that share two main regions of homology: a proline-rich domain and a carboxyl terminal domain that binds to the Arp2/3 complex.  The Arp2/3 complex initiates actin filament assembly in motile cells and formation of the immunological synapse between activated T lymphocytes and antigen-presenting cells.  WASP is a central regulator of the actin cytoskeleton in hematopoietic cells that is itself regulated by multiple signaling pathways.

The 5A5 antibody recognizes human WASP; it does not cross react with N-WASP.  It has been reported to detect WASP in lysates of hematopoietic cells and cell lines, except for neutrophils, from normal donors, but not from a group of patients having mutations of the WAS gene.

557773 Rev. 5
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
557773 Rev.5
Citations & References
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Development References (5)

  1. Burns S, Cory GO, Vainchenker W, Thrasher AJ. Mechanisms of WASp-mediated hematologic and immunologic disease. Blood. 2004; 104(12):3454-3462. (Biology).
  2. Caron E. Regulation of Wiskott-Aldrich syndrome protein and related molecules. Curr Opin Cell Biol. 2002; 14:82-87. (Biology). View Reference
  3. Kawai S, Minegishi M, Ohashi Y, et al. Flow cytometric determination of intracytoplasmic Wiskott-Aldrich syndrome protein in peripheral blood lymphocyte subpopulations. J Immunol Methods. 2002; 260:195-205. (Immunogen: Flow cytometry, Immunoprecipitation, Western blot). View Reference
  4. Orange JS, Ramesh N, Remold-O'Donnell, et al. Wiskott-Aldrich syndrome protein is required for NK cell cytotoxicity and colocalizes with actin to NK cell-activatting immunologic synapses. Proc Natl Acad Sci U S A. 2002; 99(17):11351-11356. (Clone-specific: Immunofluorescence, Western blot).
  5. Zeng R, Cannon JL, Abraham RT, et al. SLP-76 coordinates Nck-dependent Wiskott-Aldrich syndrome protein recruitment with Vav-1/Cdc42-dependent Wiskott-Aldrich syndrome protein activation at the T cell-APC contact site. J Immunol. 2003; 171:1360-1368. (Biology).
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.