Two-color flow cytometric analysis of ZBTB7B (Th-POK) expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor® 488 Mouse Anti-Human CD4 antibody (Cat. No. 557695). The cells were then washed, fixed, and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680) or PE Mouse Anti-Human ZBTB7B (ThPOK) antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B (ThPOK) [or Ig Isotype Control] versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa™ X-20 Flow Cytometer System.
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Two-color flow cytometric analysis of ZBTB7B (Th-POK) expression in human peripheral blood mononuclear cells. PBMCs were surface stained with Alexa Fluor® 488 Mouse Anti-Human CD4 antibody (Cat. No. 557695). The cells were then washed, fixed, and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680) or PE Mouse Anti-Human ZBTB7B (ThPOK) antibody. The two-color flow cytometric contour plots showing the correlated expression of ZBTB7B (ThPOK) [or Ig Isotype Control] versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR Fortessa™ X-20 Flow Cytometer System.
Product Details
BD Pharmingen™
Th-POK; THPOK; c-Krox; hcKrox
Human (QC Testing)
Mouse IgG1, κ
Human cKrox aa. 213-337
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl/test
AB_2869707
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Transcription Factor Buffer Set RUO
Size
100 Tests
Cat No.
562574
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
Alexa Fluor®488 Mouse Anti-Human CD4 RUO
Size
100 Tests
Cat No.
557695
Transcription Factor Buffer Set RUO
Size
25 Tests
Cat No.
562725
565730 Rev. 1
Antibody Details
6/hcKrox
The 6/hcKrox monoclonal antibody specifically recognizes human Th Inducing POZ-Kruppel Factor (ThPOK) which is also known as, cKrox or hcKrox. ThPOK is encoded by ZBTB7B (Zinc finger and BTB domain-containing protein 7B) and is a member of the POK/ZBT family of transcription regulators that play roles in lymphocyte development and the regulation of type I collagen genes. ThPOK expression is upregulated during the differentiation of CD4+ T helper cells but not CD8+ T cells. It indirectly increases the expression of CD4 in developing T cells by antagonizing Runx-3-mediated CD4 repression. ThPOK is also expressed in NKT and γδ T cells.
565730 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565730 Rev.1
Citations & References
View product citations for antibody "565730" on CiteAb
Development References (3)
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Heegaard AM, Gehron Robey P, Vogel W. Functional characterization of the human biglycan 5'-flanking DNA and binding of the transcription factor c-Krox. J Bone Miner Res. 1997; 12(12):2050-2060. (Biology). View Reference
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Peterkofsky B, Gosiewska A, Singh K, Pearlman S, Mahmoodian F. Species differences in cis-elements of the proalpha1(I) procollagen promoter and their binding proteins. J Cell Biochem. 1999; 73(3):408-422. (Biology). View Reference
-
Widom RL, Culic I, Lee JY, Korn JH. Cloning and characterization of hcKrox, a transcriptional regulator of extracellular matrix gene expression. Gene. 1997; 198(1-2):407-420. (Biology). View Reference
565730 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.