Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
Canada (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      PE Mouse anti-Human ILT7 (CD85g)
      PE Mouse anti-Human ILT7 (CD85g)
      Multicolor flow cytometric analysis of ILT7 expressed on human peripheral blood cells. Human peripheral blood mononuclear cells were stained with FITC Lineage Cocktail 1 (lin 1) (Cat. No. 340546 ),  APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; Left Panel) or PE Mouse Anti-Human ILT7 (CD85g) (Cat. No. 562340; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus ILT7 (or Ig Isotype Control background staining) for Lin-1 negative-gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      PE Mouse anti-Human ILT7 (CD85g)
      Multicolor flow cytometric analysis of ILT7 expressed on human peripheral blood cells. Human peripheral blood mononuclear cells were stained with FITC Lineage Cocktail 1 (lin 1) (Cat. No. 340546 ),  APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 556650; Left Panel) or PE Mouse Anti-Human ILT7 (CD85g) (Cat. No. 562340; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus ILT7 (or Ig Isotype Control background staining) for Lin-1 negative-gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      LILRA4; CD85g; ILT7; ILT-7; Immunoglobulin-like transcript 7
      Human (QC Testing)
      Mouse IgG1, κ
      ILT7–FcεRIγ-transfected 2B4 cells
      Flow cytometry (Routinely Tested)
      5 µl
      23547
      AB_10893797
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      It is recommended that cell samples be stained and maintained in the cold (4-8°C) protected from light for flow cytometric analysis.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      562340 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      17G10.2

      The 17G10.2 monoclonal antibody specifically binds to human Immunoglobulin Like Transcript 7 (ILT7), also known as CD85g. The ILT7 protein is encoded by the LILRA4 (Leukocyte Immunoglobulin Like Receptor subfamily A member 4) gene. ILT7 is a member of immunoglobulin-like transcripts (ILT) or leukocyte immunoglobulin-like receptor (LIR) gene family. ILT7 is selectively expressed on plasmacytoid dendrtic cells (pDC) but not on myeloid dendritic cells or other peripheral blood leukocytes. In response to IL-3, IL-3 receptor complex signaling downregulates ILT7 expression by pDC. ILT7 associates with the FcεRIγ signal adapter protein to form a receptor complex that can signal through activation of an immunoreceptor-based tyrosine activation motif (ITAM)-mediated signaling pathway. Signaling through the ILT7-FcεRIγ receptor complex negatively regulates the innate immune functions of pDC. ILT7 crosslinking inhibits TLR responses by pDC such as the stimulated production of type I interferon and other cytokines. Bone marrow stromal cell antigen 2 (BST2) has been identified as a biological ligand for ILT7.

      562340 Rev. 1
      Format Details
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      562340 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (3)

      1. Cao W, Bover L, Cho M, et al. Regulation of TLR7/9 responses in plasmacytoid dendritic cells by BST2 and ILT7 receptor interaction.. J Exp Med. 2009; 206(7):1603-14. (Biology). View Reference
      2. Cao W, Bover L. Signaling and ligand interaction of ILT7: receptor-mediated regulatory mechanisms for plasmacytoid dendritic cells. Immunol Rev. 2010; 234(1):163-176. (Biology). View Reference
      3. Cao W, Rosen DB, Ito T, et al. Plasmacytoid dendritic cell-specific receptor ILT7-Fc epsilonRI gamma inhibits Toll-like receptor-induced interferon production.. J Exp Med. 2006; 203(6):1399-405. (Immunogen: Bioassay, Flow cytometry, Inhibition). View Reference
      562340 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback