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Flow cytometric analysis of CD199 (CCR9) expressed on MOLT-4 cells. Human MOLT-4 cells were stained with either PE Mouse anti-Human CD199 (CCR9) antibody (Cat. No. 561607, solid line histogram) or a PE mIgG2a, κ Isotype Control (Cat. No. 553457; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
BD Pharmingen™ PE Mouse Anti-Human CD199 (CCR9)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The 112509 monoclonal antibody specifically binds to the human CCR9 chemokine receptor. CCR9 is a thymus specific CC chemokine receptor expressed at high levels by essentially all CD4+ and CD8+ T lymphocytes. CCR9 and its ligand CCL25/TECK are involved in lymphocyte recruitment in the small intestine. The immunogen used to generate the 112509 hybridoma was human CCR9-transfected cells.
Development References (4)
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Kunkel EJ, Campbell JJ, Haraldsen G, et al. Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: Epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity. J Exp Med. 2000; 192(5):761-768. (Biology). View Reference
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Kunkel EJ, Kim CH, Lazarus NH, et al. CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells.. J Clin Invest. 2003; 111(7):1001-1010. (Biology). View Reference
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Zaballos A, Gutierrez J, Varona R, Ardavin C, Marquez G. Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol. 1999; 162(10):5671-5675. (Biology). View Reference
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Zabel BA, Agace WW, Campbell JJ, et al. Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis.. J Exp Med. 1999; 190(9):1241-1256. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.