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Preparation And Storage
Recommended Assay Procedures
We have observed that the staining intensity of FITC-conjugated 15-5-5 mAb is severely reduced after fixation of stained leukocytes (≤ 1 hour with 1% formaldehyde). Therefore, one should not fix the stained cells prior to flow cytometry. We have found that freshly-isolated leukocytes and cell lines may wait for analysis in wash buffer at 4°C, without fixation, for up to 18 hours post-staining without loss of viability. Activated lymphocytes may lose viability rapidly, and data should be collected within 5 hours post-staining.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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The 15-5-5 monoclonal antibody specifically recognizes the H-2D[k] MHC class I alloantigen. It weakly cross-reacts with H-2K[d] and with cells from mice of the H-2[f] haplotype. Reactivity with other haplotypes (e.g., a, b, p, q, r, s) has not been observed.
Development References (1)
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Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980; 124(2):533-540. (Immunogen). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.