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BD Pharmingen™ FITC Mouse Anti-Human CD158a
Clone HP-3E4 (RUO)
Profile of CD158 expressed on peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
Profile of CD158 expressed on peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse BALB/c IgM
Human NK Cells
Flow cytometry (Routinely Tested)
20 µl
VI BP373, NKNK14
AB_396331
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
556062 Rev. 5
Antibody Details
HP-3E4
The HP-3E4 monoclonal antibody recognizes CD158a. CD158a is a 58 kDa membrane glycoprotein, member of the killer-inhibitory receptor (KIR). The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on <1-8% of NK cells and a subset of T cells which are involved in recognition of MHC class I molecules on target cells and inhibit cytotoxicity. CD158a is an antigen which recognizes HLA-C molecules (-Cw*0401 and -Cw*1503). This antibody is suitable for staining acetone-fixed, frozen tissue sections. It was reported that this clone also crossreacted with NKAT8 (CD158i) transfected cells.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
556062 Rev. 5
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
556062 Rev.5
Citations & References
View product citations for antibody "556062" on CiteAb
Development References (2)
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Melero I, Salmeron A, Balboa MA, Aramburu J, Lopez-Botet M. Tyrosine kinase-dependent activation of human NK cell functions upon stimulation through a 58-kDa surface antigen selectively expressed on discrete subsets of NK cells and T lymphocytes. J Immunol. 1994; 152(4):1662-1673. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
556062 Rev. 5
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.