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![BV421 Rat Anti-Mouse IgG3](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/565xxx/5658xx/565808_base/565808.png)
![BV421 Rat Anti-Mouse IgG3](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/565xxx/5658xx/565808_base/565808.png)
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Blue-Cap_Violet_test.png)
![BV421 Rat Anti-Mouse IgG3](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/565xxx/5658xx/565808_base/565808.png)
Flow cytometric analysis of CD3 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody (Rat BD Fc Block™) (Cat. No. 550270/550271). The cells were then either not incubated (background control staining; dashed line histogram) or incubated with Purified Mouse IgG3, κ Anti-Rat CD3 antibody (Cat. No. 554830/559974; solid line histogram). The cells were washed and then stained with BD Horizon™ BV421 Rat Anti-Mouse IgG3 antibody (Cat. No. 565808). The fluorescence histogram showing CD3 expression (or background control staining) was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Blue-Cap_Violet_test.png)
![BV421 Rat Anti-Mouse IgG3](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/565xxx/5658xx/565808_base/565808.png)
BD Horizon™ BV421 Rat Anti-Mouse IgG3
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Blue-Cap_Violet_test.png)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
![sampleImage/](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/buffers-and-supporting-reagents-ruo/566xxx/5663xx/566385_base/566385.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/buffers-and-supporting-reagents-ruo/563xxx/5637xx/563794_base/BD_Horizon_Brilliant_Stain_Buffer_Figure.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/cell-preparation-separation-reagents/blood-lysis/staining-and-cell-preparation/554xxx/5546xx/554656_base/554656_01.png?imwidth=320)
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![sampleImage/](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/buffers-and-supporting-reagents-ruo/563xxx/5637xx/563794_base/BD_Horizon_Brilliant_Stain_Buffer_Figure.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/cell-preparation-separation-reagents/blood-lysis/staining-and-cell-preparation/555xxx/5558xx/555899_base/35221E_555899_Figure.png?imwidth=320)
The R40-82 antibody specifically recognizes mouse IgG3 of all strains. It does not react with other Ig isotypes.
![altImg](/content/dam/bdb/products/vialimages/packaging-images/formatimages/bv421_spectrum_guide.jpg)
Development References (2)
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Duong BH, Ota T, Aït-Azzouzene D, et al. Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.. J Immunol. 2010; 184(8):4143-58. (Clone-specific: ELISA, Flow cytometry). View Reference
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Kuzin II, Snyder JE, Ugine GD, et al. Tetracyclines inhibit activated B cell function.. Int Immunol. 2001; 13(7):921-31. (Clone-specific: Flow cytometry). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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