Two-color analysis of CD16 expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 (Cat. no. 559586, both panels) and biotinylated mAb FcG7 (Right panel), followed by Streptavidin- FITC (Cat. No. 554060, both panels). Erythrocytes were lysed (PharmLyse™ Lysing Buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via- Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCaliburª (BDIS, San Jose, CA).
Two-color analysis of CD16 expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 (Cat. no. 559586, both panels) and biotinylated mAb FcG7 (Right panel), followed by Streptavidin- FITC (Cat. No. 554060, both panels). Erythrocytes were lysed (PharmLyse™ Lysing Buffer, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via- Probe™, Cat. No. 555816/555815), and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCaliburª (BDIS, San Jose, CA).
Product Details
BD Pharmingen™
Fcγ Receptor IIIAα
Pig (QC Testing)
Mouse BALB/c IgG1, κ
Pig peripheral blood leukocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_2104155
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551395 Rev. 1
Antibody Details
FcG7
The FcG7 antibody reacts with CD16, the transmembrane α chain of Fcγ Receptor III (FcγRIIIAα). In the pig, FcγRIIIAα is expressed on NK cells, monocytes, granulocytes, and pulmonary alveolar macrophages. The FcG7 antibody induces redirected cytotoxic activity of NK cells and granulocytes against tumor cells.
551395 Rev. 1
Format Details
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
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Citations & References
Development References (5)
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Aller SC, Cho D, Kim YB. Characterization of the cytolytic trigger molecules G7/PNK-E as a molecular complex on the surface of porcine phagocytes. Cell Immunol. 1995; 161(2):270-278. (Biology). View Reference
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Dato ME, Wierda WG, Kim YB. A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes. Cell Immunol. 1992; 140(2):468-477. (Immunogen: Cytotoxicity). View Reference
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Halloran PJ, Sweeney SE, Kim YB. Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7. Cell Immunol. 1994; 158(2):400-413. (Biology). View Reference
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Halloran PJ, Sweeney SE, Strohmeier CM, Kim YB. Molecular cloning and identification of the porcine cytolytic trigger molecule G7 as a Fc gamma RIII alpha (CD16) homologue. J Immunol. 1994; 153(6):2631-2641. (Biology). View Reference
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Wierda WG, Johnson BD, Dato ME, Kim YB. Induction of porcine granulocyte-mediated tumor cytotoxicity by two distinct monoclonal antibodies against lytic trigger molecules (PNK-E/G7). J Immunol. 1993; 151(12):7117-7127. (Clone-specific: Cytotoxicity). View Reference
551395 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
