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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Biotinylated 8.3 mAb may be used as a secondary reagent in immunofluorescent staining with flow cytometric analysis. Note: The isotype of clone 8.3 is mouse (SJL) IgG2a[b], κ.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The 8.3 antibody reacts specifically with mouse IgG2a of Igh-Ca and the d, e, f, g, h, j, n, and o haplotypes (eg, BALB/c, C58, and A, AKR, CBA, C3H/He, NZB). It does not react with IgG2a of Igh-Cb nor the c and p haplotypes (eg, C57BL/6, C57BL/10, SJL, DBA/1, DBA/2, SWR).
Development References (2)
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Huang CM, Parsons M, Oi VT, Huang HJ, Herzenberg LA. Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):311-321. (Clone-specific). View Reference
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Parsons M, Oi VT, Huang CM, Herzenberg LA. Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):323-334. (Clone-specific). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.