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Product Details
BD Pharmingen™
Igh-4a; Ighg1; IgG1
Mouse (QC Testing)
Mouse SJL IgG2a, κ
Not reported
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
AB_394885
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures
ELISA: Purified Mouse IgG1 (a allotype) (Cat. No. 557273) may be used as the antigen (e.g coating plates) and the Biotin Anti-Mouse IgG1[a] allotype specific antibody [MN 553500] can be used at ~ 2 µg/ml followed by Avidin-HRP (Cat. No. 554058) for detection. Investigators are highly encouraged to titrate all reagents for optimal performance.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
553500 Rev. 11
Antibody Details
10.9
This antibody is specific for mouse IgG1 of the Igh-C[a] and the c, d, e, f, g, h, n, o, and p haplotypes (e.g. BALB/c, C58, A, AKR, CBA, C3H, DBA/1, DBA/2, NZB, SWR). It has been reported not to react with IgG1 of the Igh-C[b] haplotype (e.g. C57BL/6, C57BL/10, SJL).
553500 Rev. 11
Format Details
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553500 Rev.11
Citations & References
View product citations for antibody "553500" on CiteAb
Development References (2)
-
Huang CM, Huang HJ, Lee SC. Detection of immunoglobulin heavy chain IgG3 polymorphism in wild mice with xenogeneic monoclonal antibodies. Immunogenetics. 1984; 20(5):565-575. (Biology). View Reference
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Parsons M, Oi VT, Huang CM, Herzenberg LA. Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):323-334. (Biology). View Reference
553500 Rev. 11
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.