ELISA Detection: Biotin Mouse Anti-Human IFN-γ (Cat. No. 554550) is useful as a detection antibody in a sandwich ELISA for measuring human IFN-γ protein levels. Biotin Mouse Anti-Human IFN-γ can be paired with Purified Mouse Anti-Human IFN-γ (Cat. No. 551221) as the capture antibody, with Recombinant Human IFN-γ (Cat. No. 554616/554617) as the standard. Biotin Mouse Anti-Human IFN-γ should be titrated (0.5 -2.0 µg/ml) to determine optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of human IFN-γ ranging from ~15 to 2,000 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our website, http://www.bdbiosciences.com/us/s/resources, and go to the protocols section under "ELISA and ELISPOT."
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines or chemokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human IFN-γ in serum or plasma our Human IFN-g ELISA Set (Cat. No. 555142) or Human IFN-g ELISA Kit II (Cat. No. 550612) are specially formulated and recommended.
Western Blot: The 4S.B3 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.