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BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human ILT5 (CD85a)
Clone 222821 (RUO)
Flow cytometric analysis of human ILT5 (CD85a) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human ILT5 (CD85a) antibody (Cat. No. 564469; Right Panel). Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric contour plots showing the correlated expression patterns of ILT5 (CD85a) [or Ig Isotype control staining] versus side scattered-light (SSC) characteristics were generated from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of human ILT5 (CD85a) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human ILT5 (CD85a) antibody (Cat. No. 564469; Right Panel). Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric contour plots showing the correlated expression patterns of ILT5 (CD85a) [or Ig Isotype control staining] versus side scattered-light (SSC) characteristics were generated from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of human ILT5 (CD85a) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human ILT5 (CD85a) antibody (Cat. No. 564469; Right Panel). Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric contour plots showing the correlated expression patterns of ILT5 (CD85a) [or Ig Isotype control staining] versus side scattered-light (SSC) characteristics were generated from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of human ILT5 (CD85a) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human ILT5 (CD85a) antibody (Cat. No. 564469; Right Panel). Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric contour plots showing the correlated expression patterns of ILT5 (CD85a) [or Ig Isotype control staining] versus side scattered-light (SSC) characteristics were generated from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of human ILT5 (CD85a) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human ILT5 (CD85a) antibody (Cat. No. 564469; Right Panel). Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric contour plots showing the correlated expression patterns of ILT5 (CD85a) [or Ig Isotype control staining] versus side scattered-light (SSC) characteristics were generated from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of human ILT5 (CD85a) expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human ILT5 (CD85a) antibody (Cat. No. 564469; Right Panel). Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric contour plots showing the correlated expression patterns of ILT5 (CD85a) [or Ig Isotype control staining] versus side scattered-light (SSC) characteristics were generated from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
LILRB3; HL9; LIR3; LIR-3; PIRB; PIR-B; ILT-5
Human (QC Testing)
Mouse IgG2a, κ
Human ILT5 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl/test
AB_2869583
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
100 Tests
Cat No.
557715
564469 Rev. 1
Antibody Details
222821
The 222821 monoclonal antibody specifically binds to Immunoglobulin-like transcript 5 (ILT5) which is also known as CD85a (CD85 antigen-like family member A), Leukocyte immunoglobulin-like receptor 3 (LIR-3), and Monocyte inhibitory receptor HL9. ILT5 is encoded by LILRB3 and belongs to the ILT/LTR family in the Ig gene superfamily. ILT5 is expressed by a variety of cell types including monocytes, macrophages, granulocytes, NK cells, osteoclast precursors, and a subset of T lymphocytes. This transmembrane receptor contains intracellular immunoreceptor tyrosine based inhibitory motifs (ITIMs) that can inhibit intracellular signaling. ILT5 can bind to MHC Class I molecules and appears to be involved in regulating immune responses such as NK cell-mediated cytotoxicity.
564469 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
653 nm
669 nm
564469 Rev.1
Citations & References
View product citations for antibody "564469" on CiteAb
Development References (4)
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Arm JP, Nwankwo C, Austen KF. Molecular identification of a novel family of human Ig superfamily members that possess immunoreceptor tyrosine-based inhibition motifs and homology to the mouse gp49B1 inhibitory receptor. J Immunol. 1997; 159(5):2342-2349. (Biology). View Reference
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Borges L, Hsu ML, Fanger N, Kubin M, Cosman D. A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J Immunol. 1997; 159(11):5192-5196. (Biology). View Reference
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Brown D, Trowsdale J, Allen R. The LILR family: modulators of innate and adaptive immune pathways in health and disease. Tissue Antigens. 2004; 64(3):215-225. (Biology). View Reference
-
Mori Y, Tsuji S, Inui M, et al. J Immunol. 2008; 181(7):4742-4751. (Clone-specific: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, Western blot). View Reference
564469 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.