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Human_CD248_A647_flow.png

Flow cytometric analysis of human Endosialin (CD248) on human palatal mesenchyme cells. HEPM cells (ATTC, CRL-1486) were surface stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat No. 557714, dotted line histogram) or Alexa Fluor® 647 Mouse Anti-Human Endosialin (CD248) (solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.

Human_CD248_A647_flow.png

Flow cytometric analysis of human Endosialin (CD248) on human palatal mesenchyme cells. HEPM cells (ATTC, CRL-1486) were surface stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat No. 557714, dotted line histogram) or Alexa Fluor® 647 Mouse Anti-Human Endosialin (CD248) (solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.

Human_CD248_A647_flow.png Human_CD248_A647_flow.png Red-Cap-Red-Label.jpg

Flow cytometric analysis of human Endosialin (CD248) on human palatal mesenchyme cells. HEPM cells (ATTC, CRL-1486) were surface stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat No. 557714, dotted line histogram) or Alexa Fluor® 647 Mouse Anti-Human Endosialin (CD248) (solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.

Flow cytometric analysis of human Endosialin (CD248) on human palatal mesenchyme cells. HEPM cells (ATTC, CRL-1486) were surface stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat No. 557714, dotted line histogram) or Alexa Fluor® 647 Mouse Anti-Human Endosialin (CD248) (solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
Endosialin; TEM1; CD164L1
Human (QC Testing)
Mouse IgG1, κ
Diploid human fibroblasts
Flow cytometry (Routinely Tested)
5 µl/test
AB_2739036
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  9. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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564994 Rev. 1
Antibody Details
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B1/35

The B1/35 monoclonal antibody specifically binds to CD248 which is also known as Endosialin, Tumor endothelial marker 1 (TEM1), and CD164 sialomucin-like 1 (CD164L1). CD248 is a heavily glycosylated, single-pass type I transmembrane protein. CD248 belongs to the Group XIV C-Type lectin family that includes CD93 and CD141/thrombomodulin. It is expressed on pericytes and stromal fibroblasts. Although the exact functions of CD248 remain to be determined, its expression is associated with angiogenesis and lymphoid tissue organization during development, as well as, with postnatal inflammation and tumor development and growth.

564994 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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Alexa Fluor™ 647
653 nm
669 nm
564994 Rev.1
Citations & References
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View product citations for antibody "564994" on CiteAb

Development References (4)

  1. Christian S, Ahorn H, Koehler A, et al. Molecular cloning and characterization of endosialin, a C-type lectin-like cell surface receptor of tumor endothelium. J Biol Chem. 2000; 276(10):7408-7414. (Biology). View Reference
  2. MacFadyen JR, Haworth O, Roberston D, et al. Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium. FEBS Lett. 2005; 579(12):2569-2575. (Immunogen: ELISA, Flow cytometry, Fluorescence microscopy, Immunoaffinity chromatography, Immunofluorescence, Immunoprecipitation). View Reference
  3. Rettig WJ, Garin-Chesa P, Healey JH, Su SL, Jaffe EA, Old LJ. Identification of endosialin, a cell surface glycoprotein of vascular endothelial cells in human cancer. Proc Natl Acad Sci U S A. 1992; 89(22):10832-10836. (Biology). View Reference
  4. St Croix B, Rago C, Velculescu V, et al. Genes expressed in human tumor endothelium. Science. 2000; 289(5482):1197-1202. (Biology). View Reference
View All (4) View Less
564994 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.