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Biotin Mouse Anti-Armenian and Syrian Hamster IgG Cocktail

BD Pharmingen™ Biotin Mouse Anti-Armenian and Syrian Hamster IgG Cocktail

(RUO)
Product Details
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BD Pharmingen™
0.5 mg/ml
ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry (Tested During Development)
RUO
AB_395206
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.


Description

This preparation consists of a mixture of two mouse anti-hamster IgG monoclonal antibodies. It reacts with Armenian hamster IgG1, IgG2, and IgG3, and Syrian hamster IgG1 monoclonal antibodies. It does not react with other hamster IgG groups or hamster IgM.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Biotin-conjugated mAb cocktail may be used as a secondary reagent in immunofluorescent staining. For immunohistochemical staining, we recommend the use of biotinylated anti-hamster IgG cocktail in our special formulation for immunohistochemistry, Cat. No. 550335.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
554010 Rev. 15
Components
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Description Clone Isotype EntrezGene ID
Mouse anti-Hamster IgG1 G94-56 IgG2b, κ N/A
Mouse anti-Hamster IgG2-3 G70-204 IgG1, κ N/A
554010 Rev. 15

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.